Calcium and mitochondrial metabolism in ceramide-induced cardiomyocyte death

Valentina Parra, Francisco Moraga, Jovan P Kuzmicic, Camila López-Crisosto, Rodrigo Troncoso, Natalia Torrealba, Alfredo Criollo, Jessica Díaz-Elizondo, Beverly A. Rothermel, Andrew F G Quest, Sergio Lavandero

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Ceramides are important intermediates in the biosynthesis and degradation of sphingolipids that regulate numerous cellular processes, including cell cycle progression, cell growth, differentiation and death. In cardiomyocytes, ceramides induce apoptosis by decreasing mitochondrial membrane potential and promoting cytochrome-c release. Ca2+ overload is a common feature of all types of cell death. The aim of this study was to determine the effect of ceramides on cytoplasmic Ca2+ levels, mitochondrial function and cardiomyocyte death. Our data show that C2-ceramide induces apoptosis and necrosis in cultured cardiomyocytes by a mechanism involving increased Ca2+ influx, mitochondrial network fragmentation and loss of the mitochondrial Ca2+ buffer capacity. These biochemical events increase cytosolic Ca2+ levels and trigger cardiomyocyte death via the activation of calpains.

Original languageEnglish (US)
Pages (from-to)1334-1344
Number of pages11
JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
Volume1832
Issue number8
DOIs
StatePublished - Aug 1 2013

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Ceramides
Cardiac Myocytes
Calcium
Cell Death
Apoptosis
Sphingolipids
Calpain
Mitochondrial Membrane Potential
Cytochromes c
Cell Differentiation
Cell Cycle
Buffers
Necrosis
Growth

Keywords

  • Ca
  • Cardiomyocyte
  • Cell death
  • Ceramide
  • Metabolism
  • Mitochondrial dynamics

Cite this

Parra, V., Moraga, F., Kuzmicic, J. P., López-Crisosto, C., Troncoso, R., Torrealba, N., ... Lavandero, S. (2013). Calcium and mitochondrial metabolism in ceramide-induced cardiomyocyte death. Biochimica et Biophysica Acta - Molecular Basis of Disease, 1832(8), 1334-1344. https://doi.org/10.1016/j.bbadis.2013.04.009

Calcium and mitochondrial metabolism in ceramide-induced cardiomyocyte death. / Parra, Valentina; Moraga, Francisco; Kuzmicic, Jovan P; López-Crisosto, Camila; Troncoso, Rodrigo; Torrealba, Natalia; Criollo, Alfredo; Díaz-Elizondo, Jessica; Rothermel, Beverly A.; Quest, Andrew F G; Lavandero, Sergio.

In: Biochimica et Biophysica Acta - Molecular Basis of Disease, Vol. 1832, No. 8, 01.08.2013, p. 1334-1344.

Research output: Contribution to journalArticle

Parra, V, Moraga, F, Kuzmicic, JP, López-Crisosto, C, Troncoso, R, Torrealba, N, Criollo, A, Díaz-Elizondo, J, Rothermel, BA, Quest, AFG & Lavandero, S 2013, 'Calcium and mitochondrial metabolism in ceramide-induced cardiomyocyte death', Biochimica et Biophysica Acta - Molecular Basis of Disease, vol. 1832, no. 8, pp. 1334-1344. https://doi.org/10.1016/j.bbadis.2013.04.009
Parra, Valentina ; Moraga, Francisco ; Kuzmicic, Jovan P ; López-Crisosto, Camila ; Troncoso, Rodrigo ; Torrealba, Natalia ; Criollo, Alfredo ; Díaz-Elizondo, Jessica ; Rothermel, Beverly A. ; Quest, Andrew F G ; Lavandero, Sergio. / Calcium and mitochondrial metabolism in ceramide-induced cardiomyocyte death. In: Biochimica et Biophysica Acta - Molecular Basis of Disease. 2013 ; Vol. 1832, No. 8. pp. 1334-1344.
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