Caenorhabditis elegans NPR-1-mediated behaviors are suppressed in the presence of mucoid bacteria

Kirthi C. Reddy, Ryan C. Hunter, Nikhil Bhatla, Dianne K. Newman, Dennis H. Kim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Caenorhabditis elegans exhibits a diverse range of behaviors in response to bacteria. The presence of bacterial food influences C. elegans aerotaxis, aggregation, locomotion, and pathogen avoidance behaviors through the activity of the NPR-1 neuropeptide receptor. Here, we show that mucoid strains of bacteria that produce an exopolysaccharide matrix do not induce NPR-1-dependent behaviors. In the presence of mucoid strains of bacteria, the C. elegans laboratory wild-type (WT) strain N2 exhibits behaviors characteristic of wild isolates and mutants with reduced NPR-1 activity. Specifically, N2 exhibits lawn bordering and roaming behavior on mucoid nonpathogenic bacteria and loss of pathogen avoidance on mucoid Pseudomonas aeruginosa. Alginate biosynthesis by laboratory and clinical isolates of mucoid P. aeruginosa is necessary and sufficient to attenuate NPR-1-mediated behavior and it suppresses C. elegans pathogen avoidance behavior. Our data suggest that the specific interaction with nonmucoid bacteria induces NPR-1-dependent behaviors of C. elegans. These observations provide an example of how exopolysaccharide matrix biosynthesis by a community of bacteria may inhibit specific host responses to microbes.

Original languageEnglish (US)
Pages (from-to)12887-12892
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number31
DOIs
StatePublished - Aug 2 2011

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Caenorhabditis elegans
Bacteria
Avoidance Learning
Pseudomonas aeruginosa
Neuropeptide Receptors
Locomotion
Food

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Caenorhabditis elegans NPR-1-mediated behaviors are suppressed in the presence of mucoid bacteria. / Reddy, Kirthi C.; Hunter, Ryan C.; Bhatla, Nikhil; Newman, Dianne K.; Kim, Dennis H.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 31, 02.08.2011, p. 12887-12892.

Research output: Contribution to journalArticle

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