C pigment locus mutants of the fowl produce enzymatically inactive tyrosinase‐like molecules

W. S. Oetting, A. M. Churilla, H. Yamamoto, J. A. Brumbaugh

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Three albino mutants of the fowl were tested for tyrosinase activity. Two of these mutants (c and ca) are alleles at the autosomal C locus, while the third mutant (sal) is sex‐linked. Both the standard type, E, and sal are tyrosinase positive whereas the two C mutants are tyrosinase negative. Anti‐chicken tyrosinase mouse serum was produced and all four genotypes were found to have cross‐reacting material to this antiserum. Tyrosinase from the standard type was isolated and its location on denaturing two‐dimensional gels determined. A co‐migrating series of spots was found within the protein pattern of both the standard type and the tyrosinase positive albino, sal. The same pattern of spots was also observed for c and ca with no apparent change in either the pI or the molecular weight. Transmembrane blots also showed spots that reacted with anti‐tyrosinase serum in all four genotypes and that migrated to the same location as that of standard tyrosinase. It is proposed that both c and ca are CRM+ mutants which produce tyrosinase‐like molecules that are inactive due to a change that is electrophoretically and antigenically “silent”.

Original languageEnglish (US)
Pages (from-to)237-245
Number of pages9
JournalJournal of Experimental Zoology
Issue number2
StatePublished - Aug 1985


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