C. Elegans AMPKs promote survival and arrest germline development during nutrient stress

Masamitsu Fukuyama, Kensuke Sakuma, Riyong Park, Hidefumi Kasuga, Ryotaro Nagaya, Yuriko Atsumi, Yumi Shimomura, Shinya Takahashi, Hiroaki Kajiho, Ann E Rougvie, Kenji Kontani, Toshiaki Katada

Research output: Contribution to journalArticlepeer-review

52 Scopus citations

Abstract

Mechanisms controlling development, growth, and metabolism are coordinated in response to changes in environmental conditions, enhancing the likelihood of survival to reproductive maturity. Much remains to be learned about the molecular basis underlying environmental influences on these processes. C. Elegans larvae enter a developmentally dormant state called L1 diapause when hatched into nutrientpoor conditions. The nematode pten homologue daf-18 is essential for maintenance of survival and germline stem cell quiescence during this period (Fukuyama et al., 2006; Sigmond et al., 2008), but the details of the signaling network(s) in which it functions remain to be elucidated. Here, we report that animals lacking both aak-1 and aak-2, which encode the two catalytic a subunits of AMP-activated protein kinase (AMPK), show reduced viability and failure to maintain mitotic quiescence in germline stem cells during L1 diapause. Furthermore, failure to arrest germline proliferation has a long term consequence; aak double mutants that have experienced L1 diapause develop into sterile adults when returned to food, whereas their continuously fed siblings are fertile. Both aak and daf-18 appear to maintain germline quiescence by inhibiting activity of the common downstream target, TORC1 (TOR Complex 1). In contrast, rescue of the lethality phenotype indicates that aak-2 acts not only in the intestine, as does daf-18, but also in neurons, likely promoting survival by preventing energy deprivation during L1 diapause. These results not only provide evidence that AMPK contributes to survival during L1 diapause in a manner distinct from that by which it controls dauer diapause, but they also suggest that AMPK suppresses TORC1 activity to maintain stem cell quiescence.

Original languageEnglish (US)
Pages (from-to)929-936
Number of pages8
JournalBiology Open
Volume1
Issue number10
DOIs
StatePublished - Oct 15 2012

Bibliographical note

Funding Information:
We thank Keiko Ando, Cori Bargmann, Andy Fire, Oliver Hobert, Yuji Kohara, Morris Maduro, Shohei Mitani, and Susan Strome for reagents and strains. Some strains used in this study were provided by the Caenorhabditis Genetics Center, which is funded by the National Institutes of Health, and the Mitani Laboratory at the Tokyo Women's Medical University School of Medicine (National Bioresource Project for the Experimental Animal "Nematode C. Elegans"). This work was supported by Grant-in-Aid for Young Scientists (B) from Japan Society for the Promotion of Science (JSPS), and Grants-in-Aid for Scientific Research on Innovative Areas, 'Regulatory Mechanism of Gamete Stem Cells' and Scientific Research on Priority Areas, 'G Protein Signaling' from The Ministry of Education, Culture, Sports, Science and Technology (MEXT). Work in the Rougvie lab was supported by grants from the National Science Foundation (IOB-0515682; A.R.) and American Heart Association Midwest Affiliate (M.F.).

Publisher Copyright:
© 2012 Published by The Company of Biologists Ltd.

Keywords

  • AMPK
  • Diapause
  • Stem cell

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