Butyrylcholinesterase as a marker of inflammation and liver injury in the acute and subclinical phases of canine ehrlichiosis

Guilherme M. do Carmo, Leandro Z. Crivellenti, Nathieli B. Bottari, Gustavo Machado, Sofia Borin-Crivellenti, Rafael N. Moresco, Thiago Duarte, Marta Duarte, Mirela Tinucci-Costa, Vera M. Morsch, Maria Rosa C. Schetinger, Lenita M. Stefani, Aleksandro S. Da Silva

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15 Scopus citations

Abstract

The aim of this study was to evaluate the role of butyrylcholinesterase (BChE) as a marker of inflammation and liver injury in the acute and subclinical phases of canine ehrlichiosis. Forty-two serum samples of dogs naturally infected with Ehrlichia canis were used, of which 24 were from animals with the acute phase of the disease and 18 with subclinical disease. In addition, sera from 17 healthy dogs were used as negative controls. The hematocrit, BChE activity, hepatic injury (alanine aminotransferase (ALT) and aspartate aminotransferase (AST)), nitric oxide, and cytokines levels were evaluated. The BChE activity was significantly elevated (P<0.05) in dogs with the acute phase of the disease when compared to healthy animals. However, there was a reduction on BChE activity on dogs with subclinical disease compared to the other two groups. AST and ALT levels were significantly higher (P<0.05) in the acute phase, as well as the inflammatory mediators (NOx, TNF-α, INF-γ, IL-4, IL-6) when compared to the control group. On the other hand, IL-10 levels were lower in the acute phase. Based on these results, we are able to conclude that the acute infection caused by E. canis in dogs leads to an increase on seric BChE activity and some inflammatory mediators. Therefore, this enzyme might be used as a marker of acute inflammatory response in dogs naturally infected by this bacterium.

Original languageEnglish (US)
Pages (from-to)16-21
Number of pages6
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume43
DOIs
StatePublished - Dec 1 2015

Bibliographical note

Publisher Copyright:
© 2015 Elsevier Ltd.

Keywords

  • BChE
  • Ehrlichia canis
  • Inflammation marker

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