Butyrophilin 3A1 plays an essential role in prenyl pyrophosphate stimulation of human Vγ2Vδ2 t cells

Hong Wang, Olivier Henry, Mark D Distefano, Yen Chih Wang, Johanna Raikkonen, Jukka Monkkonen, Yoshimasa Tanaka, Craig T. Morita

Research output: Contribution to journalArticle

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Abstract

Most human γδ T cells express Vγ2Vδ2 TCRs and play important roles in microbial and tumor immunity. Vγ2Vδ2 T cells are stimulated by self- and foreign prenyl pyrophosphate intermediates in isoprenoid synthesis. However, little is known about the molecular basis for this stimulation. We find that a mAb specific for butyrophilin 3 (BTN3)/CD277 Ig superfamily proteins mimics prenyl pyrophosphates. The 20.1 mAb stimulated Vγ2Vδ2 T cell clones regardless of their functional phenotype or developmental origin and selectively expanded blood Vγ2Vδ2 T cells. The γδ TCR mediates 20.1 mAb stimulation because IL-2 is released by b2 Jurkat cells transfected with Vγ2Vδ2 TCRs. 20.1 stimulation was not due to isopentenyl pyrophosphate (IPP) accumulation because 20.1 treatment of APC did not increase IPP levels. In addition, stimulation was not inhibited by statin treatment, which blocks IPP production. Importantly, small interfering RNA knockdown of BTN3A1 abolished stimulation by IPP that could be restored by re-expression of BTN3A1 but not by BTN3A2 or BTN3A3. Rhesus monkey and baboon APC presented HMBPP and 20.1 to human Vγ2Vδ2 T cells despite amino acid differences in BTN3A1 that localize to its outer surface. This suggests that the conserved inner and/or top surfaces of BTN3A1 interact with its counterreceptor. Although no binding site exists on the BTN3A1 extracellular domains, a model of the intracellular B30.2 domain predicts a basic pocket on its binding surface. However, BTN3A1 did not preferentially bind a photoaffinity prenyl pyrophosphate. Thus, BTN3A1 is required for stimulation by prenyl pyrophosphates but does not bind the intermediates with high affinity.

Original languageEnglish (US)
Pages (from-to)1029-1042
Number of pages14
JournalJournal of Immunology
Volume191
Issue number3
DOIs
StatePublished - Aug 1 2013

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T-Lymphocytes
Diphosphates
Hydroxymethylglutaryl-CoA Reductase Inhibitors
Jurkat Cells
Papio
Terpenes
Macaca mulatta
Small Interfering RNA
Interleukin-2
Immunity
Clone Cells
Binding Sites
prenyl
Butyrophilins
diphosphoric acid
Phenotype
Amino Acids
isopentenyl pyrophosphate
Neoplasms
Proteins

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Butyrophilin 3A1 plays an essential role in prenyl pyrophosphate stimulation of human Vγ2Vδ2 t cells. / Wang, Hong; Henry, Olivier; Distefano, Mark D; Wang, Yen Chih; Raikkonen, Johanna; Monkkonen, Jukka; Tanaka, Yoshimasa; Morita, Craig T.

In: Journal of Immunology, Vol. 191, No. 3, 01.08.2013, p. 1029-1042.

Research output: Contribution to journalArticle

Wang, H, Henry, O, Distefano, MD, Wang, YC, Raikkonen, J, Monkkonen, J, Tanaka, Y & Morita, CT 2013, 'Butyrophilin 3A1 plays an essential role in prenyl pyrophosphate stimulation of human Vγ2Vδ2 t cells', Journal of Immunology, vol. 191, no. 3, pp. 1029-1042. https://doi.org/10.4049/jimmunol.1300658
Wang, Hong ; Henry, Olivier ; Distefano, Mark D ; Wang, Yen Chih ; Raikkonen, Johanna ; Monkkonen, Jukka ; Tanaka, Yoshimasa ; Morita, Craig T. / Butyrophilin 3A1 plays an essential role in prenyl pyrophosphate stimulation of human Vγ2Vδ2 t cells. In: Journal of Immunology. 2013 ; Vol. 191, No. 3. pp. 1029-1042.
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