Builders, tenants, and squatters: the origins of genetic material in modern stromatolites

Victoria A. Petryshyn, Emily N. Junkins, Blake W. Stamps, Jake V. Bailey, Bradley S. Stevenson, John R. Spear, Frank A. Corsetti

Research output: Contribution to journalArticlepeer-review

Abstract

Micro-organisms have long been implicated in the construction of stromatolites. Yet, establishing a microbial role in modern stromatolite growth via molecular analysis is not always straightforward because DNA in stromatolites can have multiple origins. For example, the genomic material could represent the microbes responsible for the construction of the stromatolite (i.e., “builders”), microbes that inhabited the structure after it was built (i.e., “tenants”), or microbes/organic matter that were passively incorporated after construction from the water column or later diagenetic fluids (i.e., “squatters”). Disentangling the role of micro-organisms in stromatolite construction, already difficult in modern systems, becomes more difficult as organic signatures degrade, and their context is obscured. To evaluate our ability to accurately decipher the role of micro-organisms in stromatolite formation in geologically recent settings, 16/18S SSU rRNA gene sequences were analyzed from three systems where the context of growth was well understood: (a) an actively growing stromatolite from a silicic hot spring in Yellowstone National Park, Wyoming, where the construction of the structure is controlled by cyanobacteria; (b) a mixed carbonate and silica precipitate from Little Hot Creek, a hot spring in the Long Valley Caldera of California that has both abiogenic and biogenic components to accretion; and (c) a near-modern lacustrine carbonate stromatolite from Walker Lake, Nevada that is likely abiogenic. In all cases, the largest percentage of recovered DNA sequences, especially when focused on the deeper portions of the structures, belonged to either the tenant or squatter communities, not the actual builders. Once removed from their environmental context, correct interpretation of biology's role in stromatolite morphogenesis was difficult. Because high-throughput genomic analysis may easily lead to incorrect assumptions even in these modern and near-modern structures, caution must be exercised when interpreting micro-organismal involvement in the construction of accretionary structures throughout the rock record.

Original languageEnglish (US)
JournalGeobiology
DOIs
StateAccepted/In press - 2021

Bibliographical note

Funding Information:
This research was supported by the US National Aeronautics and Space Administration Exobiology program (grant 80NSSC19K0479), the Southern California Coastal Water Research Project, a Geological Society of America graduate student research grant, and the Agouron Institute. YNP samples were collected under Research Permit #5664 from the Yellowstone Center for Resources to J.R.S. J.R.S. is supported by the NASA Astrobiology Institute Rock Powered Life grant. B.W.S. was supported during the writing of the manuscript by the Sloan Foundation Fellowship G‐2017‐9853. This work could not have been possible without the multiyear efforts and all of the participants of the International Geobiology Summer Course from 2013 to 2016. We thank Emma Harrison, Kalen Rasmussen, and Aiyana Spear for field assistance in YNP in 2019. We thank Ann Close for keeping us from dying in a blizzard while sampling stromatolites.

Keywords

  • Little Hot Creek
  • Walker Lake
  • Yellowstone National Park
  • hot spring
  • stromatolite

PubMed: MeSH publication types

  • Journal Article

Fingerprint Dive into the research topics of 'Builders, tenants, and squatters: the origins of genetic material in modern stromatolites'. Together they form a unique fingerprint.

Cite this