A technique for single-shot, localized, water-suppressed in vivo 1H NMR with high immunity to B1 inhomogeneities is described. The single-shot localization is based on complete outer-volume suppression which is achieved with optimized, adiabatic BIR-4 pulses in combination with B0 gradient modulation, B1-insensitive water suppression is achieved with BISTRO, a train of selective adiabatic full-passage pulses interleaved with B0 gradient crushers. Computer simulations based on the Bloch equations, experimental images of the localized volume, and in vivo 1H spectroscopy experiments demonstrate the excellent spatial localization and water suppression.
Bibliographical noteFunding Information:
The Netherlands in vivo NMR facility (Bijvoet Center for Biomolecular Research, Utrecht University) is supported by the Netherlands Organization for Scientific Research (NWO). Support was also provided by Grant RR08079 from the National Institutes of Health.