Brief exposure to hyperglycemia activates dendritic cells in vitro and in vivo

Aline M. Thomas, Ying Dong, Nicholas M. Beskid, Andrés J. García, Andrew B. Adams, Julia E. Babensee

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Dendritic cells are key players in regulating immunity. These cells both activate and inhibit the immune response depending on their cellular environment. Their response to hyperglycemia, a condition common amongst diabetics wherein glucose is abnormally elevated, remains to be elucidated. In this study, the phenotype and immune response of dendritic cells exposed to hyperglycemia were characterized in vitro and in vivo using the streptozotocin-induced diabetes model. Dendritic cells were shown to be sensitive to hyperglycemia both during and after differentiation from bone marrow precursor cells. Dendritic cell behavior under hyperglycemic conditions was found to vary by phenotype, among which, tolerogenic dendritic cells were particularly sensitive. Expression of the costimulatory molecule CD86 was found to reliably increase when dendritic cells were exposed to hyperglycemia. Additionally, hydrogel-based delivery of the anti-inflammatory molecule interleukin-10 was shown to partially inhibit these effects in vivo.

Original languageEnglish (US)
Pages (from-to)5120-5129
Number of pages10
JournalJournal of cellular physiology
Volume235
Issue number6
DOIs
StatePublished - Jun 1 2020
Externally publishedYes

Bibliographical note

Funding Information:
The authors declare they do not have any conflicts of interest to disclose regarding this study. This study was supported by NIH (ACTSI, UL1TR000454; 1 R21 EB019166-01A1), Georgia Partner's Regenerative Medicine (REM) seed grant, and rgw Georgia Immunoengineering Consortium's seed grant. The first author received support from the ILET2 training grant (1 R90 DK098981, 1 T90 DK097787). Authors would like to thank Dr. Allen D. Kirk for consultation regarding immunity in diabetes.

Funding Information:
The authors declare they do not have any conflicts of interest to disclose regarding this study. This study was supported by NIH (ACTSI, UL1TR000454; 1 R21 EB019166‐01A1), Georgia Partner's Regenerative Medicine (REM) seed grant, and rgw Georgia Immunoengineering Consortium's seed grant. The first author received support from the ILET2 training grant (1 R90 DK098981, 1 T90 DK097787). Authors would like to thank Dr. Allen D. Kirk for consultation regarding immunity in diabetes.

Publisher Copyright:
© 2019 Wiley Periodicals, Inc.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Keywords

  • dendritic cells
  • diabetes
  • hydrogel
  • hyperglycemia
  • immunity
  • interleukin-10

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