Breast milk as a source for acquisition of cytomegalovirus (HCMV) in a premature infant with sepsis syndrome: Detection by real-time PCR

Benjamin T. Kerrey; Ardythe Morrow; Sheela Geraghty; Nanette Huey; Amy Sapsford; Mark R. Schleiss

doi:10.1016/j.jcv.2005.09.013

Breast milk as a source for acquisition of cytomegalovirus (HCMV) in a premature infant with sepsis syndrome: Detection by real-time PCR

Benjamin T. Kerrey, Ardythe Morrow, Sheela Geraghty, Nanette Huey, Amy Sapsford, Mark R. Schleiss

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Symptomatic disease due to human cytomegalovirus (HCMV) has been increasingly recognized in low birth weight (LBW) premature infants. Breast milk has been identified as a potential source for these infections. At approximately 60 days of life a LBW, premature infant was diagnosed with HCMV sepsis syndrome, prompting further investigation of the source for HCMV acquisition. The infant had received a combination of both fresh and frozen breast milk from his mother throughout his first 2 months of life. We utilized a real-time PCR assay for HCMV DNA to retrospectively examine viral genome copy number in serial samples of stored, frozen breast milk. This analysis indicated an increase in breast milk viral load over the first 45 days of life, heralding the HCMV sepsis syndrome that was observed clinically. Real-time PCR may be a useful tool in the evaluation of HCMV viral load in breast milk.

Original language	English (US)
Pages (from-to)	313-316
Number of pages	4
Journal	Journal of Clinical Virology
Volume	35
Issue number	3
DOIs	https://doi.org/10.1016/j.jcv.2005.09.013
State	Published - Mar 2006

Bibliographical note

Funding Information:
We acknowledge the contribution of Greg Stroup, Barbara Davidson, and the Cincinnati Children's Hospital Research Human Milk Bank. We gratefully acknowledge the guidance of Pam Groen, CCHMC Pathology Department, in the development of the real-time PCR assay. Supported by NIH N01-A125459 and HD 13021, and a Cincinnati Children's Hospital Translational Research Award.

Keywords

Breast milk
Congenital CMV infection
Cytomegalovirus
Neonatal viral infections
Premature infant
Real-time PCR
TORCH infections

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10.1016/j.jcv.2005.09.013

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title = "Breast milk as a source for acquisition of cytomegalovirus (HCMV) in a premature infant with sepsis syndrome: Detection by real-time PCR",

abstract = "Symptomatic disease due to human cytomegalovirus (HCMV) has been increasingly recognized in low birth weight (LBW) premature infants. Breast milk has been identified as a potential source for these infections. At approximately 60 days of life a LBW, premature infant was diagnosed with HCMV sepsis syndrome, prompting further investigation of the source for HCMV acquisition. The infant had received a combination of both fresh and frozen breast milk from his mother throughout his first 2 months of life. We utilized a real-time PCR assay for HCMV DNA to retrospectively examine viral genome copy number in serial samples of stored, frozen breast milk. This analysis indicated an increase in breast milk viral load over the first 45 days of life, heralding the HCMV sepsis syndrome that was observed clinically. Real-time PCR may be a useful tool in the evaluation of HCMV viral load in breast milk.",

keywords = "Breast milk, Congenital CMV infection, Cytomegalovirus, Neonatal viral infections, Premature infant, Real-time PCR, TORCH infections",

author = "Kerrey, {Benjamin T.} and Ardythe Morrow and Sheela Geraghty and Nanette Huey and Amy Sapsford and Schleiss, {Mark R.}",

note = "Funding Information: We acknowledge the contribution of Greg Stroup, Barbara Davidson, and the Cincinnati Children's Hospital Research Human Milk Bank. We gratefully acknowledge the guidance of Pam Groen, CCHMC Pathology Department, in the development of the real-time PCR assay. Supported by NIH N01-A125459 and HD 13021, and a Cincinnati Children's Hospital Translational Research Award.",

year = "2006",

month = mar,

doi = "10.1016/j.jcv.2005.09.013",

language = "English (US)",

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pages = "313--316",

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AU - Morrow, Ardythe

AU - Geraghty, Sheela

AU - Huey, Nanette

AU - Sapsford, Amy

AU - Schleiss, Mark R.

N1 - Funding Information: We acknowledge the contribution of Greg Stroup, Barbara Davidson, and the Cincinnati Children's Hospital Research Human Milk Bank. We gratefully acknowledge the guidance of Pam Groen, CCHMC Pathology Department, in the development of the real-time PCR assay. Supported by NIH N01-A125459 and HD 13021, and a Cincinnati Children's Hospital Translational Research Award.

PY - 2006/3

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N2 - Symptomatic disease due to human cytomegalovirus (HCMV) has been increasingly recognized in low birth weight (LBW) premature infants. Breast milk has been identified as a potential source for these infections. At approximately 60 days of life a LBW, premature infant was diagnosed with HCMV sepsis syndrome, prompting further investigation of the source for HCMV acquisition. The infant had received a combination of both fresh and frozen breast milk from his mother throughout his first 2 months of life. We utilized a real-time PCR assay for HCMV DNA to retrospectively examine viral genome copy number in serial samples of stored, frozen breast milk. This analysis indicated an increase in breast milk viral load over the first 45 days of life, heralding the HCMV sepsis syndrome that was observed clinically. Real-time PCR may be a useful tool in the evaluation of HCMV viral load in breast milk.

AB - Symptomatic disease due to human cytomegalovirus (HCMV) has been increasingly recognized in low birth weight (LBW) premature infants. Breast milk has been identified as a potential source for these infections. At approximately 60 days of life a LBW, premature infant was diagnosed with HCMV sepsis syndrome, prompting further investigation of the source for HCMV acquisition. The infant had received a combination of both fresh and frozen breast milk from his mother throughout his first 2 months of life. We utilized a real-time PCR assay for HCMV DNA to retrospectively examine viral genome copy number in serial samples of stored, frozen breast milk. This analysis indicated an increase in breast milk viral load over the first 45 days of life, heralding the HCMV sepsis syndrome that was observed clinically. Real-time PCR may be a useful tool in the evaluation of HCMV viral load in breast milk.

KW - Breast milk

KW - Congenital CMV infection

KW - Cytomegalovirus

KW - Neonatal viral infections

KW - Premature infant

KW - Real-time PCR

KW - TORCH infections

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Breast milk as a source for acquisition of cytomegalovirus (HCMV) in a premature infant with sepsis syndrome: Detection by real-time PCR

Abstract

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