Breast cancer cell obatoclax response characterization using passivated-electrode insulator-based dielectrophoresis

Sepeedah Soltanian-Zadeh, Kruthika Kikkeri, Ayesha N. Shajahan-Haq, Jeannine Strobl, Robert Clarke, Masoud Agah

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Inherent electrical properties of cells can be beneficial to characterize different cell lines and their response to experimental drugs. This paper presents a novel method to characterize the response of breast cancer cells to drug stimuli through use of off-chip passivated-electrode insulator-based dielectrophoresis (OπDEP) and the application of AC electric fields. This work is the first to demonstrate the ability of OπDEP to differentiate between two closely related breast cancer cell lines, LCC1 and LCC9 while assessing their drug sensitivity to an experimental anti-cancer agent, Obatoclax. Although both cell lines are derivatives of estrogen-responsive MCF-7 breast cancer cells, growth of LCC1 is estrogen independent and anti-estrogen responsive, while LCC9 is both estrogen-independent and anti-estrogen resistant. Under the same operating conditions, LCC1 and LCC9 had different DEP profiles. LCC1 cells had a trapping onset (crossover) frequency of 700 kHz and trapping efficiencies between 30–40%, while LCC9 cells had a lower crossover frequency (100 kHz) and showed higher trapping efficiencies of 40–60%. When exposed to the Obatoclax, both cell lines exhibited dose-dependent shifts in DEP crossover frequency and trapping efficiency. Here, DEP results supplemented with cell morphology and proliferation assays help us to understand the response of these breast cancer cells to Obatoclax.

Original languageEnglish (US)
Pages (from-to)1988-1995
Number of pages8
JournalELECTROPHORESIS
Volume38
Issue number16
DOIs
StatePublished - Aug 2017
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported primarily by the National Science Foundation under award number ECCS-1310090.

Publisher Copyright:
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Keywords

  • Breast cancer
  • Dielectrophoresis (DEP)
  • Drug sensitivity
  • Estrogen receptor positive (ER+)
  • GX15-070 (Obatoclax)

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