Breaking barriers: Transitioning from X-ray crystallography to cryo-EM for structural studies

Keyphrases

• Breaking Barriers 100%
• Cryogenic Electron Microscopy 100%
• X-ray Crystallography 100%
• Sample Preparation 25%
• Protein Sample 25%
• Protein Expression 25%
• Crystallization 25%
• Model Validation 25%
• Imaging Strategy 25%
• Structural Biology 25%
• Protein Complex 25%
• Model Improvement 25%
• Computational Strategies 25%
• Vitrification 25%
• Biologists 25%
• Model Building 25%
• Macromolecular Complex 25%
• Super-resolution Reconstruction 25%
• Computational Requirements 25%
• Co-purification 25%
• Protein Purification 25%
• Chaperonin GroEL 25%
• Validation Approach 25%
• Flexible Proteins 25%
• Sampling Optimization 25%
• ATPases Family 25%
• Near-atomic Resolution 25%
• Topaz 25%
• Chromatin Regulators 25%
• Single-particle Cryo-electron Microscopy 25%
• Epigenetic Signals 25%
• Biochemical Strategy 25%
• Challenges Encountered 25%
• CryoSPARC 25%
• AAA+ Domain 25%

Biochemistry, Genetics and Molecular Biology

• X-Ray Crystallography 100%
• Cryoelectron Microscopy 100%
• Copurification 40%
• Reconstruction 20%
• GroEL 20%
• AAA+ 20%
• Protein Complexes 20%
• Protein Expression 20%
• Chromatin 20%
• Vitrification 20%
• ATPase 20%
• Chaperonin 20%