Brain natriuretic peptide stimulates K and Cl secretion across porcine distal colon epithelium

T. R. Traynor, S. M. O'Grady

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Porcine distal colon epithelium was mounted in Ussing chambers and bathed with porcine Ringer solution. The serosal addition of brain natriuretic peptide (BNP; 50 nM) or atriopeptin III (AP-III; 500 nM) produced significant increases (50-75 μA/cm2) in short-circuit current (I(sc)). These increases in I(sc) were not inhibited by pretreatment with tetrodotoxin (TTX) or 5,8,11,14-eicosatetraynoic acid (ETYA). Analysis of concentration-response relationships revealed that BNP was 5.8-fold more potent than AP-III in stimulating the I(sc). BNP and AP-III significantly increased the serosal-to-mucosal (S→M) Cl flux and reduced net Cl absorption by 38 and 41%, respectively. The BNP-stimulated S→M Cl flux was abolished when HCO3 was removed. In contrast, the vasoactive intestinal peptide (VIP)-stimulated S→M Cl flux was not affected by HCO3 replacement. In addition to their effects on Cl transport, BNP and AP-III increased net Rb secretion by 79 and 58%, respectively. BNP-stimulated Rb secretion was reduced by 76% after HCO3 replacement. These results indicate that natriuretic peptides stimulate K- and HCO3-dependent Cl secretion which is not present under basal conditions or after VIP stimulation. The difference in potency between BNP and AP-III suggests that ANP-B receptors may mediate their effects on ion transport in the porcine colon.

Original languageEnglish (US)
Pages (from-to)C750-C755
JournalAmerican Journal of Physiology - Cell Physiology
Volume260
Issue number4 29-4
DOIs
StatePublished - 1991

Keywords

  • Atrial natriuretic factor-B receptor
  • Atrial natriuretic peptide
  • Bumetanide
  • Intestinal ion transport
  • Vasoactive intestinal peptide

Fingerprint Dive into the research topics of 'Brain natriuretic peptide stimulates K and Cl secretion across porcine distal colon epithelium'. Together they form a unique fingerprint.

  • Cite this