Abstract
Carbon-13 NMR spectroscopy ( 13 C MRS) offers the unique capability to measure brain metabolic rates in vivo. Hyperpolarized 13 C reduces the time required to assess brain metabolism from hours to minutes when compared with conventional 13 C MRS. This study investigates metabolism of hyperpolarized [1- 13 C]pyruvate and [2- 13 C]pyruvate in the rat brain in vivo under various anesthetics: pentobarbital, isoflurane, α-chloralose, and morphine. The apparent metabolic rate from pyruvate to lactate modeled using time courses obtained after injection of hyperpolarized [1- 13 C]pyruvate was significantly greater for isoflurane than for all other anesthetic conditions, and significantly greater for morphine than for α-chloralose. The apparent metabolic rate from pyruvate to bicarbonate was significantly greater for morphine than for all other anesthetic conditions, and significantly lower for pentobarbital than for α-chloralose. Results show that relative TCA cycle rates determined from hyperpolarized 13 C data are consistent with rates previously measured using conventional 13 C MRS under similar anesthetic conditions, and that using morphine for sedation greatly improves detection of downstream metabolic products compared with other anesthetics.
| Original language | English (US) |
|---|---|
| Article number | e4012 |
| Journal | NMR in Biomedicine |
| Volume | 31 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 1 2018 |
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Keywords
- Bicarbonate
- brain metabolism
- hyperpolarized C MRS
- lactate
PubMed: MeSH publication types
- Journal Article
- Research Support, N.I.H., Extramural
Cite this
Brain metabolism under different anesthetic conditions using hyperpolarized [1- 13 C]pyruvate and [2- 13 C]pyruvate . / Marjanska, Malgorzata; Shestov, Alexander A.; Deelchand, Dinesh K; Kittelson, Emily; Henry, Pierre-Gilles.
In: NMR in Biomedicine, Vol. 31, No. 12, e4012, 01.12.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Brain metabolism under different anesthetic conditions using hyperpolarized [1- 13 C]pyruvate and [2- 13 C]pyruvate
AU - Marjanska, Malgorzata
AU - Shestov, Alexander A.
AU - Deelchand, Dinesh K
AU - Kittelson, Emily
AU - Henry, Pierre-Gilles
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Carbon-13 NMR spectroscopy ( 13 C MRS) offers the unique capability to measure brain metabolic rates in vivo. Hyperpolarized 13 C reduces the time required to assess brain metabolism from hours to minutes when compared with conventional 13 C MRS. This study investigates metabolism of hyperpolarized [1- 13 C]pyruvate and [2- 13 C]pyruvate in the rat brain in vivo under various anesthetics: pentobarbital, isoflurane, α-chloralose, and morphine. The apparent metabolic rate from pyruvate to lactate modeled using time courses obtained after injection of hyperpolarized [1- 13 C]pyruvate was significantly greater for isoflurane than for all other anesthetic conditions, and significantly greater for morphine than for α-chloralose. The apparent metabolic rate from pyruvate to bicarbonate was significantly greater for morphine than for all other anesthetic conditions, and significantly lower for pentobarbital than for α-chloralose. Results show that relative TCA cycle rates determined from hyperpolarized 13 C data are consistent with rates previously measured using conventional 13 C MRS under similar anesthetic conditions, and that using morphine for sedation greatly improves detection of downstream metabolic products compared with other anesthetics.
AB - Carbon-13 NMR spectroscopy ( 13 C MRS) offers the unique capability to measure brain metabolic rates in vivo. Hyperpolarized 13 C reduces the time required to assess brain metabolism from hours to minutes when compared with conventional 13 C MRS. This study investigates metabolism of hyperpolarized [1- 13 C]pyruvate and [2- 13 C]pyruvate in the rat brain in vivo under various anesthetics: pentobarbital, isoflurane, α-chloralose, and morphine. The apparent metabolic rate from pyruvate to lactate modeled using time courses obtained after injection of hyperpolarized [1- 13 C]pyruvate was significantly greater for isoflurane than for all other anesthetic conditions, and significantly greater for morphine than for α-chloralose. The apparent metabolic rate from pyruvate to bicarbonate was significantly greater for morphine than for all other anesthetic conditions, and significantly lower for pentobarbital than for α-chloralose. Results show that relative TCA cycle rates determined from hyperpolarized 13 C data are consistent with rates previously measured using conventional 13 C MRS under similar anesthetic conditions, and that using morphine for sedation greatly improves detection of downstream metabolic products compared with other anesthetics.
KW - Bicarbonate
KW - brain metabolism
KW - hyperpolarized C MRS
KW - lactate
UR - http://www.scopus.com/inward/record.url?scp=85054159322&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85054159322&partnerID=8YFLogxK
U2 - 10.1002/nbm.4012
DO - 10.1002/nbm.4012
M3 - Article
C2 - 30276897
AN - SCOPUS:85054159322
VL - 31
JO - NMR in Biomedicine
JF - NMR in Biomedicine
SN - 0952-3480
IS - 12
M1 - e4012
ER -