Brain metabolism under different anesthetic conditions using hyperpolarized [1- 13 C]pyruvate and [2- 13 C]pyruvate

Malgorzata Marjanska, Alexander A. Shestov, Dinesh K Deelchand, Emily Kittelson, Pierre-Gilles Henry

Research output: Contribution to journalArticle

Abstract

Carbon-13 NMR spectroscopy ( 13 C MRS) offers the unique capability to measure brain metabolic rates in vivo. Hyperpolarized 13 C reduces the time required to assess brain metabolism from hours to minutes when compared with conventional 13 C MRS. This study investigates metabolism of hyperpolarized [1- 13 C]pyruvate and [2- 13 C]pyruvate in the rat brain in vivo under various anesthetics: pentobarbital, isoflurane, α-chloralose, and morphine. The apparent metabolic rate from pyruvate to lactate modeled using time courses obtained after injection of hyperpolarized [1- 13 C]pyruvate was significantly greater for isoflurane than for all other anesthetic conditions, and significantly greater for morphine than for α-chloralose. The apparent metabolic rate from pyruvate to bicarbonate was significantly greater for morphine than for all other anesthetic conditions, and significantly lower for pentobarbital than for α-chloralose. Results show that relative TCA cycle rates determined from hyperpolarized 13 C data are consistent with rates previously measured using conventional 13 C MRS under similar anesthetic conditions, and that using morphine for sedation greatly improves detection of downstream metabolic products compared with other anesthetics.

Original languageEnglish (US)
Article numbere4012
JournalNMR in Biomedicine
Volume31
Issue number12
DOIs
StatePublished - Dec 1 2018

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Pyruvic Acid
Metabolism
Anesthetics
Brain
Morphine
Chloralose
Isoflurane
Pentobarbital
Bicarbonates
Nuclear magnetic resonance spectroscopy
Rats
Lactic Acid
Magnetic Resonance Spectroscopy
Carbon
Injections

Keywords

  • Bicarbonate
  • brain metabolism
  • hyperpolarized C MRS
  • lactate

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural

Cite this

Brain metabolism under different anesthetic conditions using hyperpolarized [1- 13 C]pyruvate and [2- 13 C]pyruvate . / Marjanska, Malgorzata; Shestov, Alexander A.; Deelchand, Dinesh K; Kittelson, Emily; Henry, Pierre-Gilles.

In: NMR in Biomedicine, Vol. 31, No. 12, e4012, 01.12.2018.

Research output: Contribution to journalArticle

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AU - Henry, Pierre-Gilles

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