B/PI-derived synthetic peptides: synergistic effects in tethered bactericidal and endotoxin neutralizing peptides

Beulah H. Gray, Judith R. Haseman, Kevin H Mayo

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Human neutrophil bactericidal protein (B/PI) is known for its ability to kill bacteria and to neutralize the action of endotoxin. Short lininear peptides derived from residues 80-109 have been synthesized and their bactericidal and endotoxin neutralizing activities have been assayed. A series of 'walk-through' decapeptides, overlapping 3 to 4 residues, indicates that endotoxin neutralizing and partial bactericidal activities can be localized within the N- and C-terminal portions, respectively, of the 80-109 sequence. Bactericidal activity toward Pseudomonas aeruginosa was localized in central peptides of the walk-through series and greatest in peptide 90-99. By using longer peptides, residues 86-104 and 82-108, both bactericidal and endotoxin neutralizing activities are significantly enhanced. Bactericidal activity of peptide 82-108 is now only 6-fold less than that of parent B/Pl and 9-fold more potent than peptide 86-104. The 82-108 peptide was 7-fold more active at endotoxin neutralization than 86-104 but showed less enhanced activity, being approx. 470-times less active than B/PI. Cyclized 82-108 peptide retained bactericidal activity but did not improve in capacity to neutralize endotoxin.

Original languageEnglish (US)
Pages (from-to)185-190
Number of pages6
JournalBBA - General Subjects
Volume1244
Issue number1
DOIs
StatePublished - May 11 1995

Keywords

  • Antibiotic
  • B/PI
  • Bactericidal protein
  • Endotoxin neutralization
  • Peptide

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