Boundary associated long noncoding RNA mediates long-range chromosomal interactions

Ifeoma Jane Nwigwe, Yoon Jung Kim, David A. Wacker, Tae Hoon Kim, Brian P. Chadwick

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

CCCTC binding factor (CTCF) is involved in organizing chromosomes into mega base-sized, topologically associated domains (TADs) along with other factors that define sub-TAD organization. CTCF-Cohesin interactions have been shown to be critical for transcription insulation activity as it stabilizes long-range interactions to promote proper gene expression. Previous studies suggest that heterochromatin boundary activity of CTCF may be independent of Cohesin, and there may be additional mechanisms for defining topological domains. Here, we show that a boundary site we previously identified known as CTCF binding site 5 (CBS5) from the homeotic gene cluster A (HOXA) locus exhibits robust promoter activity. This promoter activity from the CBS5 boundary element generates a long noncoding RNA that we designate as boundary associated long noncoding RNA-1 (blncRNA1). Functional characterization of this RNA suggests that the transcript stabilizes long-range interactions at the HOXA locus and promotes proper expression of HOXA genes. Additionally, our functional analysis also shows that this RNA is not needed in the stabilization of CTCF-Cohesin interactions however CTCF-Cohesin interactions are critical in the transcription of blncRNA1. Thus, the CTCF-associated boundary element, CBS5, employs both Cohesin and noncoding RNA to establish and maintain topologically associated domains at the HOXA locus.

Original languageEnglish (US)
Article number0136104
JournalPloS one
Volume10
Issue number8
DOIs
StatePublished - Aug 24 2015

Fingerprint

Long Noncoding RNA
binding sites
Insulator Elements
loci
Binding Sites
Transcription
transcription (genetics)
promoter regions
gene expression
Genes
homeotic genes
insulating materials
RNA
heterochromatin
Gene Expression
messenger RNA
Untranslated RNA
Functional analysis
multigene family
Heterochromatin

Cite this

Boundary associated long noncoding RNA mediates long-range chromosomal interactions. / Nwigwe, Ifeoma Jane; Kim, Yoon Jung; Wacker, David A.; Kim, Tae Hoon; Chadwick, Brian P.

In: PloS one, Vol. 10, No. 8, 0136104, 24.08.2015.

Research output: Contribution to journalArticle

Nwigwe, Ifeoma Jane ; Kim, Yoon Jung ; Wacker, David A. ; Kim, Tae Hoon ; Chadwick, Brian P. / Boundary associated long noncoding RNA mediates long-range chromosomal interactions. In: PloS one. 2015 ; Vol. 10, No. 8.
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