Colorectal cancer (CRC) is an intractable intestinal tumor. Cancer cells prefer to aerobic glycolysis to meet the requirements of rapid proliferation. Hence, discovery of a bioactive molecule cold inhibit aerobic glycolysis, which is regarded as a effective strategies for prevention and treatment of CRC. In this study, we first discovered a foxtail millet bran-derived bound polyphenol (BPIS) with anti-proliferative activity in HT-29 cell (colon cancer cell lines) and its nude mice model. The data further indicated the anti-CRC active ingredients and molecular mechanisms of BPIS. Here we revealed that BPIS could act anti-proliferative activity in HT-29 cells depending on reversing aerobic glycolysis, and this effect was achieved by up-regulation of miR-149 expression by BPIS, which directly targeted the 3′-UTR of c-myc, thereby inhibiting PKM2-mediated aerobic glycolysis. Therefore, BPIS might be used as a new inhibitor of glycolysis for enhancing intestines health benefits.
Bibliographical noteFunding Information:
This study was supported by the National Natural Science Foundation of China (Nos. 31770382 , 81803238 , 31500630 ), “1331 project” Collaborative Innovation Center ( 1331 CIC ) and Collaborative Innovation Team ( 1331 CIT ), The Key Project of Shanxi Province (No. 201801D111001 ), the Key Project of the Key Research and Development Program of Shanxi Province , China (No. 201603D21106-01 ).
- Anti-colorectal cancer
- Foxtail millet bran