Bordetella pertussis FbpA binds both unchelated iron and iron siderophore complexes

Sambuddha Banerjee, Aruna J. Weerasinghe, Claire J. Parker Siburt, R. Timothy Kreulen, Sandra K. Armstrong, Timothy J. Brickman, Lisa A. Lambert, Alvin L. Crumbliss

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Bordetella pertussis is the causative agent of whooping cough. This pathogenic bacterium can obtain the essential nutrient iron using its native alcaligin siderophore and by utilizing xeno-siderophores such as desferrioxamine B, ferrichrome, and enterobactin. Previous genome-wide expression profiling identified an iron repressible B. pertussis gene encoding a periplasmic protein (FbpABp). A previously reported crystal structure shows significant similarity between FbpABp and previously characterized bacterial iron binding proteins, and established its iron-binding ability. Bordetella growth studies determined that FbpABp was required for utilization of not only unchelated iron, but also utilization of iron bound to both native and xeno-siderophores. In this in vitro solution study, we quantified the binding of unchelated ferric iron to FbpABp in the presence of various anions and importantly, we demonstrated that FbpABp binds all the ferric siderophores tested (native and xeno) with μM affinity. In silico modeling augmented solution data. FbpABp was incapable of iron removal from ferric xeno-siderophores in vitro. However, when FbpABp was reacted with native ferric-alcaligin, it elicited a pronounced change in the iron coordination environment, which may signify an early step in FbpA Bp-mediated iron removal from the native siderophore. To our knowledge, this is the first time the periplasmic component of an iron uptake system has been shown to bind iron directly as Fe3+ and indirectly as a ferric siderophore complex.

Original languageEnglish (US)
Pages (from-to)3952-3960
Number of pages9
JournalBiochemistry
Volume53
Issue number24
DOIs
StatePublished - Jun 24 2014

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