Boosting of SARS-CoV-2 immunity in nonhuman primates using an oral rhabdoviral vaccine

Kah Whye Peng, Timothy Carey, Patrycja Lech, Rianna Vandergaast, Miguel Muñoz-Alía, Nandakumar Packiriswamy, Clement Gnanadurai, Karina Y Krotova, Mulu Tesfay, Christopher Ziegler, Michelle Haselton, Kara Sevola, Chase Lathrum, Samantha Reiter, Riya Narjari, Baskar Balakrishnan, Lukkana Suksanpaisan, Toshie Sakuma, Jordan Recker, Lianwen ZhangScott Waniger, Luke Russell, Christopher D. Petro, Christos A. Kyratsous, Alina Baum, Jody L Janecek, Rachael M. Lee, Sabarinathan Ramachandran, Melanie L. Graham, Steven J Russell

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

An orally active vaccine capable of boosting SARS-CoV-2 immune responses in previously infected or vaccinated individuals would help efforts to achieve and sustain herd immunity. Unlike mRNA-loaded lipid nanoparticles and recombinant replication-defective adenoviruses, replicating vesicular stomatitis viruses with SARS-CoV-2 spike glycoproteins (VSV-SARS2) were poorly immunogenic after intramuscular administration in clinical trials. Here, by G protein trans-complementation, we generated VSV-SARS2(+G) virions with expanded target cell tropism. Compared to parental VSV-SARS2, G-supplemented viruses were orally active in virus-naive and vaccine-primed cynomolgus macaques, powerfully boosting SARS-CoV-2 neutralizing antibody titers. Clinical testing of this oral VSV-SARS2(+G) vaccine is planned.

Original languageEnglish (US)
Pages (from-to)2342-2351
Number of pages10
JournalVaccine
Volume40
Issue number15
DOIs
StatePublished - Apr 1 2022

Bibliographical note

Funding Information:
We gratefully acknowledge the excellent and expert contributions of Carlen Hill, Jody Janecek, Ruby Klish, Rachael Lee, Christian Moses, Brenna Mulhollam, Lucas Mutch, Melanie Niewinski, Sierra Palmer, Scott Oppler, Jordan Truell for husbandry and clinical care of our animals, Mikayla Chavis, Sarah Gresch, Laura Hocum Stone for in vitro studies, Meghan Moore and Timothy O'Brien for pathology, and Mellani Lubaug, Margret Tavai-Tuisalo'o, Jade Wilder for administrative support at the University of Minnesota's Preclinical Research Center. We gratefully acknowledge the excellent and expert support of Parthasarathy Rangarajan, Naoya Sato, Anna Tran, Ravi Maisuria, Amar Singh, and Janice Anoka for sample processing and specimen archiving.

Publisher Copyright:
© 2021

Keywords

  • Neutralizing antibodies
  • Oral vaccine
  • Rhabdovirus
  • SARS-CoV-2
  • T cells
  • VSV

Fingerprint

Dive into the research topics of 'Boosting of SARS-CoV-2 immunity in nonhuman primates using an oral rhabdoviral vaccine'. Together they form a unique fingerprint.

Cite this