Bone resorption in dogs with calcium oxalate urolithiasis and idiopathic hypercalciuria

Austin C. Luskin, Jody P Lulich, Sarah C. Gresch, Eva Furrow

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

People with calcium oxalate (CaOx) urolithiasis and idiopathic hypercalciuria (IH) often have evidence of increased bone resorption, but bone turnover has not previously been investigated in dogs with these conditions. The aim of this study was to determine whether a marker of bone resorption, β-crosslaps, differs between dogs with CaOx urolithiasis and IH compared to controls. This retrospective, cross-sectional study used a canine specific ELISA to measure β-crosslaps concentrations in stored frozen serum samples from 20 dogs with CaOx urolithiasis and IH and 20 breed-, sex-, and age-matched stone-free controls (18 Miniature Schnauzers, 14 Bichons Frise, and 8 Shih Tzus). Dogs with CaOx urolithiasis and IH had lower β-crosslaps concentrations relative to controls (P =.0043), and β-crosslaps had a moderate negative correlation with urinary calcium-to-creatinine ratios (r = −0.44, P =.0044). Miniature Schnauzers had lower β-crosslaps concentrations than the other two breeds (P =.0035). The ELISA had acceptable intra-assay precision, but concentrations decreased when samples were repeatedly assayed over time. Assay recovery rates were also below acceptance criteria. In conclusion, Miniature Schnauzers, Bichons Frise, and Shih Tzus with CaOx urolithiasis and IH have evidence of decreased bone resorption compared to stone-free controls. This suggests that other causes of IH, such as intestinal hyperabsorption of calcium, underlie risk for CaOx urolithiasis in these breeds. Results should be confirmed in larger populations and with other β-crosslaps assays and additional biomarkers of bone turnover. The stability of canine serum β-crosslaps after freeze-thaw cycles and storage at various temperatures requires investigation.

Original languageEnglish (US)
Pages (from-to)129-134
Number of pages6
JournalResearch in veterinary science
Volume123
DOIs
StatePublished - Apr 1 2019

Fingerprint

glutamyl-lysyl-alanyl-histidyl-aspartyl-glycyl-glycyl-arginine
Hypercalciuria
calcium oxalate
bone resorption
Bone Resorption
Dogs
dogs
breeds
Bone Remodeling
assays
Canidae
bones
enzyme-linked immunosorbent assay
calcium
Enzyme-Linked Immunosorbent Assay
Calcium
freeze-thaw cycles
cross-sectional studies
creatinine
Calcium Oxalate Nephrolithiasis

Keywords

  • Bone resorption
  • Calcium oxalate
  • Dogs
  • Hypercalciuria
  • Urolithiasis

PubMed: MeSH publication types

  • Journal Article

Cite this

Bone resorption in dogs with calcium oxalate urolithiasis and idiopathic hypercalciuria. / Luskin, Austin C.; Lulich, Jody P; Gresch, Sarah C.; Furrow, Eva.

In: Research in veterinary science, Vol. 123, 01.04.2019, p. 129-134.

Research output: Contribution to journalArticle

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abstract = "People with calcium oxalate (CaOx) urolithiasis and idiopathic hypercalciuria (IH) often have evidence of increased bone resorption, but bone turnover has not previously been investigated in dogs with these conditions. The aim of this study was to determine whether a marker of bone resorption, β-crosslaps, differs between dogs with CaOx urolithiasis and IH compared to controls. This retrospective, cross-sectional study used a canine specific ELISA to measure β-crosslaps concentrations in stored frozen serum samples from 20 dogs with CaOx urolithiasis and IH and 20 breed-, sex-, and age-matched stone-free controls (18 Miniature Schnauzers, 14 Bichons Frise, and 8 Shih Tzus). Dogs with CaOx urolithiasis and IH had lower β-crosslaps concentrations relative to controls (P =.0043), and β-crosslaps had a moderate negative correlation with urinary calcium-to-creatinine ratios (r = −0.44, P =.0044). Miniature Schnauzers had lower β-crosslaps concentrations than the other two breeds (P =.0035). The ELISA had acceptable intra-assay precision, but concentrations decreased when samples were repeatedly assayed over time. Assay recovery rates were also below acceptance criteria. In conclusion, Miniature Schnauzers, Bichons Frise, and Shih Tzus with CaOx urolithiasis and IH have evidence of decreased bone resorption compared to stone-free controls. This suggests that other causes of IH, such as intestinal hyperabsorption of calcium, underlie risk for CaOx urolithiasis in these breeds. Results should be confirmed in larger populations and with other β-crosslaps assays and additional biomarkers of bone turnover. The stability of canine serum β-crosslaps after freeze-thaw cycles and storage at various temperatures requires investigation.",
keywords = "Bone resorption, Calcium oxalate, Dogs, Hypercalciuria, Urolithiasis",
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