Bone marrow transplantation across major histocompatibility barriers in mice. IV. Graft-versus-host disease in TLI-conditioned mice

Daniel A. Vallera; Alexandra Filipovich; Christine C.B. Soderling; John H. Kersey

doi:10.1016/0090-1229(82)90128-3

Bone marrow transplantation across major histocompatibility barriers in mice. IV. Graft-versus-host disease in TLI-conditioned mice

Daniel A. Vallera
, Alexandra Filipovich
, Christine C.B. Soderling
, John H. Kersey

Radiation Oncology

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

We studied the effect of transplanting lymphohematopoietic grafts across major histocompatibility barriers into mice conditioned with either single dose 900 rad total body irradiation (TBI), fractionated 17 × 200 rad total lymphoid irradiation (TLI, or single dose 900 rad TLI plus cyclophosphamide (TLI + CY). In all three conditioning regimens, survival following BALB c → C57BL 6 grafting was inversely related to the number of immunocompetent T cells in the donor graft. These findings show that regardless of conditioning protocol (TBI or TLI), it was not possible to protect mice from lethal GvHD when the number of T cells in donor grafts approximated those found in patient bone marrow aspirates. When T cells were eliminated from the donor graft by pretreatment with monoclonal anti-Thy 1.2 plus complement prior to injection into TBI-conditioned recipients, long-term protection from lethal GvHD was indeed possible.

Original language	English (US)
Pages (from-to)	437-447
Number of pages	11
Journal	Clinical Immunology and Immunopathology
Volume	23
Issue number	2
DOIs	https://doi.org/10.1016/0090-1229(82)90128-3
State	Published - May 1982

Bibliographical note

Funding Information:
...T his article is dedicated to Robert A. Good on the occasion of his 60th birthday. I This work was supported in part by National Cancer Institute Grants POl-CA-15548, CA-24794 and ROl-CA31618 the Pediatric Oncology Program Project POI-CA21737, University of Minnesota Graduate School Grant 488-0325-4909-02, and the Hubert H. Humphrey Cancer Fund. 2 Abbreviations used: TLI, total lymphoid irradiation; TBI, total body irradiation; BMS, bone marrow plus spleen; CY, cyclophosphamide; GvHD, graft-versus-host disease; C, ComPlement: BM, bone marrow: PBM, peripheral blood mononuclear cell; FITC, fluorescein isothiocyanate conjugated.

Publisher link

10.1016/0090-1229(82)90128-3

Find It

Find It at U of M Libraries

Cite this

@article{cdc020f72c0f4f808607fe2ea21cb78e,

title = "Bone marrow transplantation across major histocompatibility barriers in mice. IV. Graft-versus-host disease in TLI-conditioned mice",

abstract = "We studied the effect of transplanting lymphohematopoietic grafts across major histocompatibility barriers into mice conditioned with either single dose 900 rad total body irradiation (TBI), fractionated 17 × 200 rad total lymphoid irradiation (TLI, or single dose 900 rad TLI plus cyclophosphamide (TLI + CY). In all three conditioning regimens, survival following BALB c → C57BL 6 grafting was inversely related to the number of immunocompetent T cells in the donor graft. These findings show that regardless of conditioning protocol (TBI or TLI), it was not possible to protect mice from lethal GvHD when the number of T cells in donor grafts approximated those found in patient bone marrow aspirates. When T cells were eliminated from the donor graft by pretreatment with monoclonal anti-Thy 1.2 plus complement prior to injection into TBI-conditioned recipients, long-term protection from lethal GvHD was indeed possible.",

author = "Vallera, \{Daniel A.\} and Alexandra Filipovich and Soderling, \{Christine C.B.\} and Kersey, \{John H.\}",

note = "Funding Information: ...T his article is dedicated to Robert A. Good on the occasion of his 60th birthday. I This work was supported in part by National Cancer Institute Grants POl-CA-15548, CA-24794 and ROl-CA31618 the Pediatric Oncology Program Project POI-CA21737, University of Minnesota Graduate School Grant 488-0325-4909-02, and the Hubert H. Humphrey Cancer Fund. 2 Abbreviations used: TLI, total lymphoid irradiation; TBI, total body irradiation; BMS, bone marrow plus spleen; CY, cyclophosphamide; GvHD, graft-versus-host disease; C, ComPlement: BM, bone marrow: PBM, peripheral blood mononuclear cell; FITC, fluorescein isothiocyanate conjugated.",

year = "1982",

month = may,

doi = "10.1016/0090-1229(82)90128-3",

language = "English (US)",

volume = "23",

pages = "437--447",

journal = "Clinical Immunology and Immunopathology",

issn = "0090-1229",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Bone marrow transplantation across major histocompatibility barriers in mice. IV. Graft-versus-host disease in TLI-conditioned mice

AU - Vallera, Daniel A.

AU - Filipovich, Alexandra

AU - Soderling, Christine C.B.

AU - Kersey, John H.

N1 - Funding Information: ...T his article is dedicated to Robert A. Good on the occasion of his 60th birthday. I This work was supported in part by National Cancer Institute Grants POl-CA-15548, CA-24794 and ROl-CA31618 the Pediatric Oncology Program Project POI-CA21737, University of Minnesota Graduate School Grant 488-0325-4909-02, and the Hubert H. Humphrey Cancer Fund. 2 Abbreviations used: TLI, total lymphoid irradiation; TBI, total body irradiation; BMS, bone marrow plus spleen; CY, cyclophosphamide; GvHD, graft-versus-host disease; C, ComPlement: BM, bone marrow: PBM, peripheral blood mononuclear cell; FITC, fluorescein isothiocyanate conjugated.

PY - 1982/5

Y1 - 1982/5

N2 - We studied the effect of transplanting lymphohematopoietic grafts across major histocompatibility barriers into mice conditioned with either single dose 900 rad total body irradiation (TBI), fractionated 17 × 200 rad total lymphoid irradiation (TLI, or single dose 900 rad TLI plus cyclophosphamide (TLI + CY). In all three conditioning regimens, survival following BALB c → C57BL 6 grafting was inversely related to the number of immunocompetent T cells in the donor graft. These findings show that regardless of conditioning protocol (TBI or TLI), it was not possible to protect mice from lethal GvHD when the number of T cells in donor grafts approximated those found in patient bone marrow aspirates. When T cells were eliminated from the donor graft by pretreatment with monoclonal anti-Thy 1.2 plus complement prior to injection into TBI-conditioned recipients, long-term protection from lethal GvHD was indeed possible.

AB - We studied the effect of transplanting lymphohematopoietic grafts across major histocompatibility barriers into mice conditioned with either single dose 900 rad total body irradiation (TBI), fractionated 17 × 200 rad total lymphoid irradiation (TLI, or single dose 900 rad TLI plus cyclophosphamide (TLI + CY). In all three conditioning regimens, survival following BALB c → C57BL 6 grafting was inversely related to the number of immunocompetent T cells in the donor graft. These findings show that regardless of conditioning protocol (TBI or TLI), it was not possible to protect mice from lethal GvHD when the number of T cells in donor grafts approximated those found in patient bone marrow aspirates. When T cells were eliminated from the donor graft by pretreatment with monoclonal anti-Thy 1.2 plus complement prior to injection into TBI-conditioned recipients, long-term protection from lethal GvHD was indeed possible.

UR - https://www.scopus.com/pages/publications/0019954401

UR - https://www.scopus.com/pages/publications/0019954401#tab=citedBy

U2 - 10.1016/0090-1229(82)90128-3

DO - 10.1016/0090-1229(82)90128-3

M3 - Article

C2 - 7049469

AN - SCOPUS:0019954401

SN - 0090-1229

VL - 23

SP - 437

EP - 447

JO - Clinical Immunology and Immunopathology

JF - Clinical Immunology and Immunopathology

IS - 2

ER -

Bone marrow transplantation across major histocompatibility barriers in mice. IV. Graft-versus-host disease in TLI-conditioned mice

Abstract

Bibliographical note

Publisher link

Find It

Other files and links

Fingerprint

Cite this