Bone marrow transplantation across major histocompatibility barriers in mice. IV. Graft-versus-host disease in TLI-conditioned mice

Daniel A. Vallera, Alexandra Filipovich, Christine C.B. Soderling, John H. Kersey

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

We studied the effect of transplanting lymphohematopoietic grafts across major histocompatibility barriers into mice conditioned with either single dose 900 rad total body irradiation (TBI), fractionated 17 × 200 rad total lymphoid irradiation (TLI, or single dose 900 rad TLI plus cyclophosphamide (TLI + CY). In all three conditioning regimens, survival following BALB c → C57BL 6 grafting was inversely related to the number of immunocompetent T cells in the donor graft. These findings show that regardless of conditioning protocol (TBI or TLI), it was not possible to protect mice from lethal GvHD when the number of T cells in donor grafts approximated those found in patient bone marrow aspirates. When T cells were eliminated from the donor graft by pretreatment with monoclonal anti-Thy 1.2 plus complement prior to injection into TBI-conditioned recipients, long-term protection from lethal GvHD was indeed possible.

Original languageEnglish (US)
Pages (from-to)437-447
Number of pages11
JournalClinical Immunology and Immunopathology
Volume23
Issue number2
DOIs
StatePublished - May 1982

Bibliographical note

Funding Information:
...T his article is dedicated to Robert A. Good on the occasion of his 60th birthday. I This work was supported in part by National Cancer Institute Grants POl-CA-15548, CA-24794 and ROl-CA31618 the Pediatric Oncology Program Project POI-CA21737, University of Minnesota Graduate School Grant 488-0325-4909-02, and the Hubert H. Humphrey Cancer Fund. 2 Abbreviations used: TLI, total lymphoid irradiation; TBI, total body irradiation; BMS, bone marrow plus spleen; CY, cyclophosphamide; GvHD, graft-versus-host disease; C, ComPlement: BM, bone marrow: PBM, peripheral blood mononuclear cell; FITC, fluorescein isothiocyanate conjugated.

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

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