Bone marrow-derived epithelial cells and hair follicle stem cells contribute to development of chronic cutaneous neoplasms

Heuijoon Park, Sonali Lad, Kelsey Boland, Kelly Johnson, Nyssa Readio, Guangchun Jin, Samuel Asfaha, Kelly S. Patterson, Ashok Singh, Xiangdong Yang, Douglas Londono, Anupama Singh, Carol Trempus, Derek Gordon, Timothy C. Wang, Rebecca J. Morris

Research output: Contribution to journalArticle

Abstract

We used allogeneic bone marrow transplantation (BMT) and a mouse multistage cutaneous carcinogenesis model to probe recruitment of bone marrow-derived epithelial cells (BMDECs) in skin tumors initiated with the carcinogen, dimethylbenz[a]anthracene (DMBA), and promoted with 12-O-tetradecanolyphorbol-13-acetate (TPA). BMDECs clustered in the lesional epithelium, expressed cytokeratins, proliferated, and stratified. We detected cytokeratin induction in plastic-adherent bone marrow cells (BMCs) cultured in the presence of filter-separated keratinocytes (KCs) and bone morphogenetic protein 5 (BMP5). Lineage-depleted BMCs migrated towards High Mobility Group Box 1 (HMGB1) protein and epidermal KCs in ex vivo invasion assays. Naive female mice receiving BMTs from DMBA-treated donors developed benign and malignant lesions after TPA promotion alone. We conclude that BMDECs contribute to the development of papillomas and dysplasia, demonstrating a systemic contribution to these lesions. Furthermore, carcinogen-exposed BMCs can initiate benign and malignant lesions upon tumor promotion. Ultimately, these findings may suggest targets for treatment of non-melanoma skin cancers.

Original languageEnglish (US)
Article number5293
JournalNature Communications
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2018

Fingerprint

neoplasms
Hair Follicle
bone marrow
stem cells
hair
Skin Neoplasms
Stem cells
Bone Marrow Cells
9,10-Dimethyl-1,2-benzanthracene
Bone
Stem Cells
Bone Marrow
Epithelial Cells
Keratins
Keratinocytes
Carcinogens
Bone Morphogenetic Protein 5
Acetates
HMGB1 Protein
Skin

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

Cite this

Bone marrow-derived epithelial cells and hair follicle stem cells contribute to development of chronic cutaneous neoplasms. / Park, Heuijoon; Lad, Sonali; Boland, Kelsey; Johnson, Kelly; Readio, Nyssa; Jin, Guangchun; Asfaha, Samuel; Patterson, Kelly S.; Singh, Ashok; Yang, Xiangdong; Londono, Douglas; Singh, Anupama; Trempus, Carol; Gordon, Derek; Wang, Timothy C.; Morris, Rebecca J.

In: Nature Communications, Vol. 9, No. 1, 5293, 01.12.2018.

Research output: Contribution to journalArticle

Park, H, Lad, S, Boland, K, Johnson, K, Readio, N, Jin, G, Asfaha, S, Patterson, KS, Singh, A, Yang, X, Londono, D, Singh, A, Trempus, C, Gordon, D, Wang, TC & Morris, RJ 2018, 'Bone marrow-derived epithelial cells and hair follicle stem cells contribute to development of chronic cutaneous neoplasms' Nature Communications, vol. 9, no. 1, 5293. https://doi.org/10.1038/s41467-018-07688-8
Park, Heuijoon ; Lad, Sonali ; Boland, Kelsey ; Johnson, Kelly ; Readio, Nyssa ; Jin, Guangchun ; Asfaha, Samuel ; Patterson, Kelly S. ; Singh, Ashok ; Yang, Xiangdong ; Londono, Douglas ; Singh, Anupama ; Trempus, Carol ; Gordon, Derek ; Wang, Timothy C. ; Morris, Rebecca J. / Bone marrow-derived epithelial cells and hair follicle stem cells contribute to development of chronic cutaneous neoplasms. In: Nature Communications. 2018 ; Vol. 9, No. 1.
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