Blood outgrowth endothelial cells alter remodeling of completely biological engineered grafts implanted into the sheep femoral artery

Lee A. Meier, Zeeshan H. Syedain, Matthew T. Lahti, Sandra S. Johnson, Minna H. Chen, Robert P. Hebbel, Robert T. Tranquillo

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Hemocompatibility of tissue-engineered vascular grafts remains a major hurdle to clinical utility for small-diameter grafts. Here we assessed the feasibility of using autologous blood outgrowth endothelial cells to create an endothelium via lumenal seeding on completely biological, decellularized engineered allografts prior to implantation in the sheep femoral artery. The 4-mm-diameter, 2- to 3-cm-long grafts were fabricated from fibrin gel remodeled into an aligned tissue tube in vitro by ovine dermal fibroblasts prior to decellularization. Decellularized grafts pre-seeded with blood outgrowth endothelial cells (n=3) retained unprecedented (>95 %) monolayer coverage 1 h post-implantation and had greater endothelial coverage, smaller wall thickness, and more basement membrane after 9-week implantation, including a final week without anti-coagulation therapy, compared with contralateral non-seeded controls. These results support the use of autologous blood outgrowth endothelial cells as a viable source of endothelial cells for creating an endothelium with biological function on decellularized engineered allografts made from fibroblast-remodeled fibrin.

Original languageEnglish (US)
Pages (from-to)242-249
Number of pages8
JournalJournal of cardiovascular translational research
Volume7
Issue number2
DOIs
StatePublished - Jan 1 2014

Fingerprint

Femoral Artery
Sheep
Endothelial Cells
Transplants
Fibrin
Endothelium
Allografts
Fibroblasts
Blood Vessel Prosthesis
Basement Membrane
Gels
Skin
Therapeutics

Keywords

  • Endothelial cells
  • Tissue engineering
  • Vascular graft

Cite this

Blood outgrowth endothelial cells alter remodeling of completely biological engineered grafts implanted into the sheep femoral artery. / Meier, Lee A.; Syedain, Zeeshan H.; Lahti, Matthew T.; Johnson, Sandra S.; Chen, Minna H.; Hebbel, Robert P.; Tranquillo, Robert T.

In: Journal of cardiovascular translational research, Vol. 7, No. 2, 01.01.2014, p. 242-249.

Research output: Contribution to journalArticle

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