Blocking primers reduce co-amplification of plant DNA when studying bacterial endophyte communities

Research output: Contribution to journalArticle

  • 2 Citations

Abstract

A blocking primer set based on the technique described by Vestheim and Jarman (2008) was developed to reduce amplification of non-target plant DNA when conducting metagenomic studies on bacterial endophyte communities. Bacterial amplification efficiency was increased 300-fold compared to standard PCR in an Illumina-based study of Sorghastrum nutans leaves.

Original languageEnglish (US)
Pages (from-to)1-3
Number of pages3
JournalJournal of Microbiological Methods
Volume117
DOIs
StatePublished - Oct 1 2015

Fingerprint

Plant DNA
Endophytes
Metagenomics
Polymerase Chain Reaction

Keywords

  • Blocking
  • Chloroplast
  • Endophyte
  • Metagenomics
  • Mitochondria

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

MeSH PubMed subject areas

  • Journal Article
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

Cite this

@article{70c1d57a3691496dafdfcab408b2c8e4,
title = "Blocking primers reduce co-amplification of plant DNA when studying bacterial endophyte communities",
abstract = "A blocking primer set based on the technique described by Vestheim and Jarman (2008) was developed to reduce amplification of non-target plant DNA when conducting metagenomic studies on bacterial endophyte communities. Bacterial amplification efficiency was increased 300-fold compared to standard PCR in an Illumina-based study of Sorghastrum nutans leaves.",
keywords = "Blocking, Chloroplast, Endophyte, Metagenomics, Mitochondria",
author = "Arenz, {Brett E.} and Schlatter, {Dan C.} and Bradeen, {James M.} and Kinkel, {Linda L.}",
year = "2015",
month = "10",
doi = "10.1016/j.mimet.2015.07.003",
volume = "117",
pages = "1--3",
journal = "Journal of Microbiological Methods",
issn = "0167-7012",
publisher = "Elsevier",

}

TY - JOUR

T1 - Blocking primers reduce co-amplification of plant DNA when studying bacterial endophyte communities

AU - Arenz,Brett E.

AU - Schlatter,Dan C.

AU - Bradeen,James M.

AU - Kinkel,Linda L.

PY - 2015/10/1

Y1 - 2015/10/1

N2 - A blocking primer set based on the technique described by Vestheim and Jarman (2008) was developed to reduce amplification of non-target plant DNA when conducting metagenomic studies on bacterial endophyte communities. Bacterial amplification efficiency was increased 300-fold compared to standard PCR in an Illumina-based study of Sorghastrum nutans leaves.

AB - A blocking primer set based on the technique described by Vestheim and Jarman (2008) was developed to reduce amplification of non-target plant DNA when conducting metagenomic studies on bacterial endophyte communities. Bacterial amplification efficiency was increased 300-fold compared to standard PCR in an Illumina-based study of Sorghastrum nutans leaves.

KW - Blocking

KW - Chloroplast

KW - Endophyte

KW - Metagenomics

KW - Mitochondria

UR - http://www.scopus.com/inward/record.url?scp=84969389689&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84969389689&partnerID=8YFLogxK

U2 - 10.1016/j.mimet.2015.07.003

DO - 10.1016/j.mimet.2015.07.003

M3 - Article

VL - 117

SP - 1

EP - 3

JO - Journal of Microbiological Methods

T2 - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

ER -