Blocking primers reduce co-amplification of plant DNA when studying bacterial endophyte communities

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A blocking primer set based on the technique described by Vestheim and Jarman (2008) was developed to reduce amplification of non-target plant DNA when conducting metagenomic studies on bacterial endophyte communities. Bacterial amplification efficiency was increased 300-fold compared to standard PCR in an Illumina-based study of Sorghastrum nutans leaves.

Original languageEnglish (US)
Pages (from-to)1-3
Number of pages3
JournalJournal of Microbiological Methods
Volume117
DOIs
StatePublished - Oct 1 2015

Keywords

  • Blocking
  • Chloroplast
  • Endophyte
  • Metagenomics
  • Mitochondria

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