Black soybean seed coat polyphenols prevent AAPH-nduced oxidative DNA-damage in HepG2 cells

Yasukiyo Yoshioka, Xiu Li, Tianshun Zhang, Takakazu Mitani, Michiko Yasuda, Fumio Nanba, Toshiya Toda, Yoko Yamashita, Hitoshi Ashida

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


Black soybean seed coat, extract (BE), which contains abundant polyphenols such as procyanidins, cyanidin 3-glucoside, (+)-catechin, and (-)-epicatechin, has been reported on health beneficial functions such as antioxidant activity, anti-inflammatory, anti-obesity, and anti-diabetic activities. In this study, we investigated that prevention of BE and its polyphenols on 2,2'-azobis(2-methylpropionamide) dihydrochloride (AAPH)-induced oxidative DNA damage, and found that these polyphenols inhibited AAPH-induced formation of 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a biomarker for oxidative DNA damage in HepG2 cells. Under the same conditions, these polyphenols also inhibited AAPH-induced accumulation of reactive oxygen species (ROS) in the cells. Inhibition of ROS accumulation was observed in both cytosol and nucleus. It was confirmed that these polyphenols inhibited formation of AAPH radical using oxygen radical absorbance capacity assay under the cell-free conditions. These results indicate that polyphenols in BE inhibit free radical-induced oxidative DNA damages by their potent antioxidant activity. Thus, BE is an effective food material for prevention of oxidative stress and oxidative DNA damages.

Original languageEnglish (US)
Pages (from-to)108-114
Number of pages7
JournalJournal of Clinical Biochemistry and Nutrition
Issue number2
StatePublished - 2017

Bibliographical note

Publisher Copyright:
© 2017 JCBN.


  • 8-OHdG
  • Hepatocytes
  • LC-MS/MS
  • Procyanidin
  • ROS


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