The protozoan, Tetrahymena thermophila, metabolizes pentachloronitrobenzene to several products, including nitrite, pentachlorothioanisole and pentachloroaniline. The latter two metabolites were identified by gas chromatographymass spectrometry. Pentachlorothioanisole may be produced via a glutathione-dependent pathway, and two key enzymes of this pathway, glutathione transferase and thiol S-methyltransferase, have been detected in crude extracts of this microorganism.
Bibliographical noteFunding Information:
We thank Dr. Robert Barkley for running was supported by grants from the University Creative Work, and the National Institutes the GC-MS analyses. of Colorado Council of Health (ES 02639).