Biosynthesis of plant-speciWc phenylpropanoids by construction of an artiWcial biosynthetic pathway in Escherichia coli

Oksik Choi, Cheng Zhu Wu, Sun Young Kang, Jong Seog Ahn, Tai Boong Uhm, Young Soo Hong

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Biological synthesis of plant secondary metabolites has attracted increasing attention due to their proven or assumed beneWcial properties and health-promoting eVects. Phenylpropanoids are the precursors to a range of important plant metabolites such as the secondary metabolites belonging to the Xavonoid/stilbenoid class of compounds. In this study, engineered Escherichia coli containing artiWcial phenylpropanoid biosynthetic pathways utilizing tyrosine as the initial precursor were established for production of plant-speciWc metabolites such as ferulic acid, naringenin, and resveratrol. The construction of the artiWcial pathway utilized tyrosine ammonia lyase and 4-coumarate 3-hydroxylase from Saccharothrix espanaensis, cinnamate/4-coumarate: coenzyme A ligase from Streptomyces coelicolor, caVeic acid O-methyltransferase and chalcone synthase from Arabidopsis thaliana, and stilbene synthase from Arachis hypogaea.

Original languageEnglish (US)
Pages (from-to)1657-1665
Number of pages9
JournalJournal of Industrial Microbiology and Biotechnology
Issue number10
StatePublished - Oct 2011

Bibliographical note

Funding Information:
This work was supported in part by the 21C Frontier Microbial Genomics and Application Center, Basic Science Research Program, and Global R&D Center program, the Ministry of Science and Technology, Republic of Korea, and by a grant from KRIBB Research Initiative Program. The authors would also like to thank Joon-Tae Park for assistance in the LC–MS experiment.


  • ArtiWcial pathway
  • Biosynthesis
  • Heterologous expression
  • Phenylpropanoid


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