Biological synthesis of plant secondary metabolites has attracted increasing attention due to their proven or assumed beneWcial properties and health-promoting eVects. Phenylpropanoids are the precursors to a range of important plant metabolites such as the secondary metabolites belonging to the Xavonoid/stilbenoid class of compounds. In this study, engineered Escherichia coli containing artiWcial phenylpropanoid biosynthetic pathways utilizing tyrosine as the initial precursor were established for production of plant-speciWc metabolites such as ferulic acid, naringenin, and resveratrol. The construction of the artiWcial pathway utilized tyrosine ammonia lyase and 4-coumarate 3-hydroxylase from Saccharothrix espanaensis, cinnamate/4-coumarate: coenzyme A ligase from Streptomyces coelicolor, caVeic acid O-methyltransferase and chalcone synthase from Arabidopsis thaliana, and stilbene synthase from Arachis hypogaea.
|Original language||English (US)|
|Number of pages||9|
|Journal||Journal of Industrial Microbiology and Biotechnology|
|State||Published - Oct 2011|
- ArtiWcial pathway
- Heterologous expression