Biosynthesis of plant-speciWc phenylpropanoids by construction of an artiWcial biosynthetic pathway in Escherichia coli

Oksik Choi, Cheng Zhu Wu, Sun Young Kang, Jong Seog Ahn, Tai Boong Uhm, Young Soo Hong

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

Biological synthesis of plant secondary metabolites has attracted increasing attention due to their proven or assumed beneWcial properties and health-promoting eVects. Phenylpropanoids are the precursors to a range of important plant metabolites such as the secondary metabolites belonging to the Xavonoid/stilbenoid class of compounds. In this study, engineered Escherichia coli containing artiWcial phenylpropanoid biosynthetic pathways utilizing tyrosine as the initial precursor were established for production of plant-speciWc metabolites such as ferulic acid, naringenin, and resveratrol. The construction of the artiWcial pathway utilized tyrosine ammonia lyase and 4-coumarate 3-hydroxylase from Saccharothrix espanaensis, cinnamate/4-coumarate: coenzyme A ligase from Streptomyces coelicolor, caVeic acid O-methyltransferase and chalcone synthase from Arabidopsis thaliana, and stilbene synthase from Arachis hypogaea.

Original languageEnglish (US)
Pages (from-to)1657-1665
Number of pages9
JournalJournal of Industrial Microbiology and Biotechnology
Volume38
Issue number10
DOIs
StatePublished - Oct 2011

Keywords

  • ArtiWcial pathway
  • Biosynthesis
  • Heterologous expression
  • Phenylpropanoid

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