Biosynthesis of peptidoglycan in the one million molecular weight range by membrane preparations from Bacillus megaterium

W. P. Schrader, B. E. Beckman, M. M. Beckman, J. S. Anderson, D. P. Fan

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Peptidoglycan made in vitro by membrane preparations of Bacillus megaterium had a buoyant density in CsCl different from that of native cell wall. This newly made material was not covalently attached to substantial amounts of previously formed cell material such as wall. From sedimentation velocity centrifugation, the molecular weight of much of the in vitro product was found to be greater than 6x10 6. Peptidoglycan made in the presence of penicillin had smaller molecular weights suggesting that transpeptidation was in part responsible for the large size of the product. With reaction conditions in which transpeptidation was presumed to have occurred, release of alanine was observed. This release was inhibited by penicillin.

Original languageEnglish (US)
Pages (from-to)4807-4814
Number of pages8
JournalJournal of Biological Chemistry
Volume249
Issue number15
StatePublished - 1974

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