Biomolecular resource utilization in elementary cell-free gene circuits

Dan Siegal-Gaskins, Vincent Noireaux, Richard M. Murray

Research output: Chapter in Book/Report/Conference proceedingConference contribution

14 Scopus citations

Abstract

We present a detailed dynamical model of the behavior of transcription-translation circuits in vitro that makes explicit the roles played by essential molecular resources. A set of simple two-gene test circuits operating in a cell-free biochemical 'breadboard' validate this model and highlight the consequences of limited resource availability. In particular, we are able to confirm the existence of biomolecular 'crosstalk' and isolate its individual sources. The implications of crosstalk for biomolecular circuit design and function are discussed.

Original languageEnglish (US)
Title of host publication2013 American Control Conference, ACC 2013
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1531-1536
Number of pages6
ISBN (Print)9781479901777
DOIs
StatePublished - 2013
Event2013 1st American Control Conference, ACC 2013 - Washington, DC, United States
Duration: Jun 17 2013Jun 19 2013

Publication series

NameProceedings of the American Control Conference
ISSN (Print)0743-1619

Other

Other2013 1st American Control Conference, ACC 2013
Country/TerritoryUnited States
CityWashington, DC
Period6/17/136/19/13

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