Biomarkers of oxidative stress study V: Ozone exposure of rats and its effect on lipids, proteins, and DNA in plasma and urine

Maria B. Kadiiska, Samar Basu, Nathan Brot, Christopher Cooper, A. Saari Csallany, Michael J. Davies, Magdalene M. George, Dennis M. Murray, L. Jackson Roberts, Mark K. Shigenaga, Rajindar S. Sohal, Roland Stocker, David H. Van Thiel, Ingrid Wiswedel, Gary E. Hatch, Ronald P. Mason

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Ozone exposure effect on free radical-catalyzed oxidation products of lipids, proteins, and DNA in the plasma and urine of rats was studied as a continuation of the international Biomarker of Oxidative Stress Study (BOSS) sponsored by NIEHS/NIH. The goal was to identify a biomarker for ozone-induced oxidative stress and to assess whether inconsistent results often reported in the literature might be due to the limitations of the available methods for measuring the various types of oxidative products. The time- and dose-dependent effects of ozone exposure on rat plasma lipid hydroperoxides, malondialdehyde, F2-isoprostanes, protein carbonyls, methionine oxidation, and tyrosine- and phenylalanine oxidation products, as well as urinary malondialdehyde and F2-isoprostanes were investigated with various techniques. The criterion used to recognize a marker in the model of ozone exposure was that a significant effect could be identified and measured in a biological fluid seen at both doses at more than one time point. No statistically significant differences between the experimental and the control groups at either ozone dose and time point studied could be identified in this study. Tissue samples were not included. Despite all the work accomplished in the BOSS study of ozone, no available product of oxidation in biological fluid has yet met the required criteria of being a biomarker. The current negative findings as a consequence of ozone exposure are of great importance, because they document that in complex systems, as the present in vivo experiment, the assays used may not provide meaningful data of ozone oxidation, especially in human studies.

Original languageEnglish (US)
Pages (from-to)408-415
Number of pages8
JournalFree Radical Biology and Medicine
StatePublished - 2013

Bibliographical note

Funding Information:
The research was supported by the Intramural Program of the NIH, National Institute of Environmental Health Sciences. The research described in this article has been reviewed by the National Health and Environmental Effects Research Laboratory, United States Environmental Protection Agency and approved for publication. Approval does not signify that the contents necessarily reflect the views and policies of the agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use.

Copyright 2013 Elsevier B.V., All rights reserved.


  • Biomarkers
  • Oxidative stress
  • Ozone exposure
  • Rats

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