TY - GEN
T1 - Biomarker quantification in unprocessed human sera by using a new nanomagnetic protein assay
AU - Li, Yuanpeng
AU - Jing, Ying
AU - Srinivasan, Balasubramanian
AU - Yao, Xiaofeng
AU - Hugger, Marie A.
AU - Xing, Chengguo
AU - Wang, Jianping
PY - 2010
Y1 - 2010
N2 - Early recognition and prevention of chronic disease, such as lung cancer, require a fast, accurate detection and longitudinal monitoring on potential biomarkers, which could identify the molecule change in the initial stage of the chronic disease. Here we report the realization of specific and accurate quantification of a low-abundance serum protein in unprocessed human sera, using our novel giant magnetoresistive (GMR) biosensing system with uniform high-magnetic-moment nanoparticles and a competition based detection scheme. Only one antibody is needed for such detection scheme. The quantification of interleukin-6 (IL-6, a low-abundance protein and a potential cancer biomarker), as low as 125 fM IL-6 proteins, directly in 4 μL of unprocessed human sera was demonstrated within 5 minutes by such system. The results nicely differentiate normal individuals and lung cancer patients. This platform has great potential to facilitate the identification and validation of disease biomarkers.
AB - Early recognition and prevention of chronic disease, such as lung cancer, require a fast, accurate detection and longitudinal monitoring on potential biomarkers, which could identify the molecule change in the initial stage of the chronic disease. Here we report the realization of specific and accurate quantification of a low-abundance serum protein in unprocessed human sera, using our novel giant magnetoresistive (GMR) biosensing system with uniform high-magnetic-moment nanoparticles and a competition based detection scheme. Only one antibody is needed for such detection scheme. The quantification of interleukin-6 (IL-6, a low-abundance protein and a potential cancer biomarker), as low as 125 fM IL-6 proteins, directly in 4 μL of unprocessed human sera was demonstrated within 5 minutes by such system. The results nicely differentiate normal individuals and lung cancer patients. This platform has great potential to facilitate the identification and validation of disease biomarkers.
KW - Giant Magnetoresistance
KW - Magnetic biosensor
KW - biomarker
KW - protein assay
UR - http://www.scopus.com/inward/record.url?scp=79251588719&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79251588719&partnerID=8YFLogxK
U2 - 10.1063/1.3530018
DO - 10.1063/1.3530018
M3 - Conference contribution
AN - SCOPUS:79251588719
SN - 9780735408661
T3 - AIP Conference Proceedings
SP - 237
EP - 243
BT - 8th International Conference on the Scientific and Clinical Applications of Magnetic Carriers
T2 - 8th International Conference on the Scientific and Clinical Applications of Magnetic Carriers
Y2 - 25 May 2010 through 29 May 2010
ER -
