Biologically Active Polypeptides and Hydrolytic Enzymes in Sea Anemones of Northern Temperate Waters

E. V. Klyshko, A. P. Il'ina, M. M. Monastyrnaya, Yu V. Burtseva, E. E. Kostina, T. A. Zykova, N. I. Menzorova, E. P. Kozlovskaya

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The content of biologically active polypeptides in aqueous and ethanol extracts of seven sea anemone species collected near Sakhalin Island (Sea of Okhotsk) and in Posyet Bay (Sea of Japan) was analyzed. Water extracts of the sea anemone Cribrinopsis similis showed the highest hemolytic activity, while ethanol extracts proved to have toxic properties. The levels of toxic and hemolytic activity of extracts of sea anemones inhabiting northern temperate waters were 2 to 3 orders of magnitude lower, compared to tropic species. The reason for this is likely to be the differences in the habitat conditions and biological traits of these animals. The water extracts of all species possessed proteolytic, phospholipase A2, and low DNAase activities, except Actinostola sp., whose aqueous extract contained a high activity alkaline DNAase. The species studied contained a wide range of proteinase inhibitors, O-glycosyl hydrolases (glycosidases and polysaccharide hydrolases). Water extracts of C. similis and Stomphia coccinea possessed the highest laminarinase activity. High activity of N-galactopyranosidase was found in water extracts of S. coccinea and Oulactis orientalis.

Original languageEnglish (US)
Pages (from-to)161-166
Number of pages6
JournalRussian Journal of Marine Biology
Volume29
Issue number3
DOIs
StatePublished - May 2003
Externally publishedYes

Bibliographical note

Funding Information:
ACKNOWLEDGMENTS The authors sincerely thank S.D. Grebel’nyi (Zoological Institute, Russian Academy of Sciences, St. Petersburg) for help in identification of species and M.B. Ivanova for valuable advice. This study was supported by grants from the Russian Foundation for Basic Research (no. 02-04-49486) and from the Ministry of Education of the Russian Federation under the project US CRDF (no. REC-003).

Keywords

  • Aytolysins
  • DNAases
  • Glycosidases
  • Hydrolases
  • Neurotoxins
  • Phospholipases
  • Proteinase inhibitors
  • Proteinases
  • Sea anemones
  • Sea of Japan
  • Sea of Okhotsk

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