Abstract
The content of biologically active polypeptides in aqueous and ethanol extracts of seven sea anemone species collected near Sakhalin Island (Sea of Okhotsk) and in Posyet Bay (Sea of Japan) was analyzed. Water extracts of the sea anemone Cribrinopsis similis showed the highest hemolytic activity, while ethanol extracts proved to have toxic properties. The levels of toxic and hemolytic activity of extracts of sea anemones inhabiting northern temperate waters were 2 to 3 orders of magnitude lower, compared to tropic species. The reason for this is likely to be the differences in the habitat conditions and biological traits of these animals. The water extracts of all species possessed proteolytic, phospholipase A2, and low DNAase activities, except Actinostola sp., whose aqueous extract contained a high activity alkaline DNAase. The species studied contained a wide range of proteinase inhibitors, O-glycosyl hydrolases (glycosidases and polysaccharide hydrolases). Water extracts of C. similis and Stomphia coccinea possessed the highest laminarinase activity. High activity of N-galactopyranosidase was found in water extracts of S. coccinea and Oulactis orientalis.
Original language | English (US) |
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Pages (from-to) | 161-166 |
Number of pages | 6 |
Journal | Russian Journal of Marine Biology |
Volume | 29 |
Issue number | 3 |
DOIs | |
State | Published - May 2003 |
Externally published | Yes |
Bibliographical note
Funding Information:ACKNOWLEDGMENTS The authors sincerely thank S.D. Grebel’nyi (Zoological Institute, Russian Academy of Sciences, St. Petersburg) for help in identification of species and M.B. Ivanova for valuable advice. This study was supported by grants from the Russian Foundation for Basic Research (no. 02-04-49486) and from the Ministry of Education of the Russian Federation under the project US CRDF (no. REC-003).
Keywords
- Aytolysins
- DNAases
- Glycosidases
- Hydrolases
- Neurotoxins
- Phospholipases
- Proteinase inhibitors
- Proteinases
- Sea anemones
- Sea of Japan
- Sea of Okhotsk