Biological variability of lipoprotein-associated phospholipase A2 activity in healthy individuals

Leslie J. Donato, Jeffrey W. Meeusen, Sarah M. Jenkins, Stacy J. Hartman, Amy K Saenger, Nikola A. Baumann, Darci R. Block, Allan S. Jaffe

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Objectives Measuring lipoprotein-associated phospholipase A2 (Lp-PLA2) activity can aid in identifying individuals at higher risk of coronary heart disease. However, the biological variation of Lp-PLA2 activity and corresponding reference change value (RCV) is unknown which limits interpretation of results. In this study we aim to define the intra- and inter-individual variability of Lp-PLA2 activity in a healthy reference population. Methods A total of 24 healthy individuals (22–47 years of age) were prospectively collected at several time points: daily for five days (after overnight fast), daily for three days (while non-fasting), weekly for four weeks (after overnight fast), and monthly for 6 months (after overnight fast). Intra-individual and inter-individual variability was determined. The index of individuality (IoI) and reference change value (RCV) were calculated for each time period. Results Variability in Lp-PLA2 activity was not different in fasting versus non-fasting states and also did not change in daily versus monthly testing. The RCV for monthly Lp-PLA2 activity was found to be 17%. More than 90% of the variability was attributable to between person differences while analytic variability comprised < 9% of the variability and within-person variability was low at < 0.7%. The index of individuality for monthly testing was 0.30 Conclusions In a healthy population, Lp-PLA2 activity displays low analytical and within-person variability and higher inter-individual variability. The change required to differentiate a true change in patient status was determined to be 17% for monthly measurements. The between individual variability and corresponding RCV for the activity assay are lower than previously reported results for the Lp-PLA2 mass assay.

Original languageEnglish (US)
Pages (from-to)347-349
Number of pages3
JournalClinical Biochemistry
Volume50
Issue number6
DOIs
StatePublished - Apr 2017

Keywords

  • Index of individuality
  • Lipoprotein-associated phospholipase A
  • Lp-PLA
  • PLAC activity
  • Platelet activating factor acetylhydrolase
  • RCV
  • Reference change value

PubMed: MeSH publication types

  • Comparative Study
  • Journal Article

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