Biological properties of allogenic articular chondrocytes on the surface of bovine cartilage explants in vitro

G. Kim, M. Okumura, D. Bosnakovski, T. Ishiguro, T. Kadosawa, T. Fujinaga

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Bovine cartilage explants were co-cultured with or without allogenic chondrocytes for 4 weeks. The attachment of the applied chondrocytes to cartilage after labelling with fluorescence was assessed using a confocal laser microscope. Morphological changes and the production of extracellular matrix (ECM) of co-cultured chondrocytes on intact and damaged surfaces of cartilage were evaluated by histological and immunohistochemical methods. Co-cultured chondrocytes attached to and proliferated on the intact and damaged areas of cartilage, and a new layer was created there. The defects were also filled with ECM produced by the co-cultured chondrocytes. Glycosaminoglycans and collagen type II were detected in the newly formed ECM, and large numbers of rounded chondrocytes were observed at primitive lacunae in this matrix at 4 weeks of culture. The results suggest that chondrocytes have the ability to attach to, to proliferate on and to establish a new matrix on the intact and damaged surfaces of cartilage explants.

Original languageEnglish (US)
Pages (from-to)418-423
Number of pages6
JournalJournal of Veterinary Medicine Series A: Physiology Pathology Clinical Medicine
Volume50
Issue number8
DOIs
StatePublished - Oct 2003
Externally publishedYes

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