Biological effect of silver-modified nanostructured titanium dioxide in cancer

Nefeli Lagopati, Athanassios Kotsinas, Dimitris Veroutis, Konstantinos Evangelou, Angelos Papaspyropoulos, Michalis Arfanis, Polycarpos Falaras, Paraskevi V. Kitsiou, Ioannis Pateras, Anna Bergonzini, Teresa Frisan, Spyridon Kyriazis, Dimitrios S. Tsoukleris, Effie Photini C. Tsilibary, Maria Gazouli, Evangelia A. Pavlatou, Vassilis G. Gorgoulis

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Background/Aim: Nanomedicine is a promising scientific field that exploits the unique properties of innovative nanomaterials, providing alternative solutions in diagnostics, prevention and therapeutics. Titanium dioxide nanoparticles (TiO2NPs) have a great spectrum of photocatalytic antibacterial and anticancer applications. The chemical modification of TiO2optimizes its bioactive performance. The aim of this study was the development of silver modified NPs (Ag/TiO2NPs) with anticancer potential. Materials and Methods: Ag/TiO2NPs were prepared through the sol-gel method, were fully characterized and were tested on cultured breast cancer epithelial cells (MCF-7 and MDAMB-231). The MTT colorimetric assay was used to estimate cellular viability. Western blot analysis of protein expression along with a DNA-laddering assay were employed for apoptosis detection. Results and Conclusion: We show that photo-activated Ag/TiO2NPs exhibited significant cytotoxicity on the highly malignant MDA-MB-231 cancer cells, inducing apoptosis, while MCF-7 cells that are characterized by low invasive properties were unaffected under the same conditions.

Original languageEnglish (US)
Pages (from-to)425-439
Number of pages15
JournalCancer Genomics and Proteomics
Issue number3 Suppl
StatePublished - 2021

Bibliographical note

Funding Information:
This study was financially supported by the National Public Investment Program of the Ministry of Development and Investment/General Secretariat for Research and Technology, in the framework of the of the Flagship Initiative to address SARS-CoV-2 (2020ΣΕ01300001). It was also supported by the Welfare Foundation for Social & Cultural Sciences, Athens, Greece (KIKPE); the Hellenic Foundation for Research and Innovation (HFRI) grants no. 775 and 3782 and NKUA-SARG grant 70/3/8916, by the kind donation of H. Pappas and by IKY scholarships program (action “Reinforcement of Postdoctoral Researchers”, (N. Lagopati-scholarship for Postdoctoral Researchers-MIS5001552).

Publisher Copyright:
© 2021 International Institute of Anticancer Research. All rights reserved.


  • Anticancer properties
  • Apoptosis
  • Cytotoxicity
  • Nanomedicine
  • Nanostructured titanium dioxide
  • Photocatalysis
  • Silvermodified titanium dioxide
  • Nanostructures/chemistry
  • Humans
  • Nanomedicine/methods
  • Silver/chemistry
  • Titanium/pharmacology
  • Neoplasms/chemically induced

PubMed: MeSH publication types

  • Journal Article


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