Biological effect of silver-modified nanostructured titanium dioxide in cancer

Nefeli Lagopati; Athanassios Kotsinas; Dimitris Veroutis; Konstantinos Evangelou; Angelos Papaspyropoulos; Michalis Arfanis; Polycarpos Falaras; Paraskevi V. Kitsiou; Ioannis Pateras; Anna Bergonzini; Teresa Frisan; Spyridon Kyriazis; Dimitrios S. Tsoukleris; Effie Photini C. Tsilibary; Maria Gazouli; Evangelia A. Pavlatou; Vassilis G. Gorgoulis

doi:10.21873/CGP.20269

Biological effect of silver-modified nanostructured titanium dioxide in cancer

Nefeli Lagopati, Athanassios Kotsinas, Dimitris Veroutis, Konstantinos Evangelou, Angelos Papaspyropoulos, Michalis Arfanis, Polycarpos Falaras, Paraskevi V. Kitsiou, Ioannis Pateras, Anna Bergonzini, Teresa Frisan, Spyridon Kyriazis, Dimitrios S. Tsoukleris, Effie Photini C. Tsilibary, Maria Gazouli, Evangelia A. Pavlatou, Vassilis G. Gorgoulis

Neuroscience

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Background/Aim: Nanomedicine is a promising scientific field that exploits the unique properties of innovative nanomaterials, providing alternative solutions in diagnostics, prevention and therapeutics. Titanium dioxide nanoparticles (TiO₂NPs) have a great spectrum of photocatalytic antibacterial and anticancer applications. The chemical modification of TiO₂optimizes its bioactive performance. The aim of this study was the development of silver modified NPs (Ag/TiO₂NPs) with anticancer potential. Materials and Methods: Ag/TiO₂NPs were prepared through the sol-gel method, were fully characterized and were tested on cultured breast cancer epithelial cells (MCF-7 and MDAMB-231). The MTT colorimetric assay was used to estimate cellular viability. Western blot analysis of protein expression along with a DNA-laddering assay were employed for apoptosis detection. Results and Conclusion: We show that photo-activated Ag/TiO₂NPs exhibited significant cytotoxicity on the highly malignant MDA-MB-231 cancer cells, inducing apoptosis, while MCF-7 cells that are characterized by low invasive properties were unaffected under the same conditions.

Original language	English (US)
Pages (from-to)	425-439
Number of pages	15
Journal	Cancer Genomics and Proteomics
Volume	18
Issue number	3 Suppl
DOIs	https://doi.org/10.21873/CGP.20269
State	Published - 2021

Bibliographical note

Funding Information:
This study was financially supported by the National Public Investment Program of the Ministry of Development and Investment/General Secretariat for Research and Technology, in the framework of the of the Flagship Initiative to address SARS-CoV-2 (2020ΣΕ01300001). It was also supported by the Welfare Foundation for Social & Cultural Sciences, Athens, Greece (KIKPE); the Hellenic Foundation for Research and Innovation (HFRI) grants no. 775 and 3782 and NKUA-SARG grant 70/3/8916, by the kind donation of H. Pappas and by IKY scholarships program (action “Reinforcement of Postdoctoral Researchers”, (N. Lagopati-scholarship for Postdoctoral Researchers-MIS5001552).

Publisher Copyright:
© 2021 International Institute of Anticancer Research. All rights reserved.

Keywords

Anticancer properties
Apoptosis
Cytotoxicity
Nanomedicine
Nanostructured titanium dioxide
Photocatalysis
Silvermodified titanium dioxide
Nanostructures/chemistry
Humans
Nanomedicine/methods
Silver/chemistry
Titanium/pharmacology
Neoplasms/chemically induced

PubMed: MeSH publication types

Journal Article

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.21873/CGP.20269

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Lagopati, N., Kotsinas, A., Veroutis, D., Evangelou, K., Papaspyropoulos, A., Arfanis, M., Falaras, P., Kitsiou, P. V., Pateras, I., Bergonzini, A., Frisan, T., Kyriazis, S., Tsoukleris, D. S., Tsilibary, E. P. C., Gazouli, M., Pavlatou, E. A., & Gorgoulis, V. G. (2021). Biological effect of silver-modified nanostructured titanium dioxide in cancer. Cancer Genomics and Proteomics, 18(3 Suppl), 425-439. https://doi.org/10.21873/CGP.20269

Lagopati, N, Kotsinas, A, Veroutis, D, Evangelou, K, Papaspyropoulos, A, Arfanis, M, Falaras, P, Kitsiou, PV, Pateras, I, Bergonzini, A, Frisan, T, Kyriazis, S, Tsoukleris, DS, Tsilibary, EPC, Gazouli, M, Pavlatou, EA & Gorgoulis, VG 2021, 'Biological effect of silver-modified nanostructured titanium dioxide in cancer', Cancer Genomics and Proteomics, vol. 18, no. 3 Suppl, pp. 425-439. https://doi.org/10.21873/CGP.20269

@article{ebff5cef32b94e27b2e7879bd2a7aba0,

title = "Biological effect of silver-modified nanostructured titanium dioxide in cancer",

abstract = "Background/Aim: Nanomedicine is a promising scientific field that exploits the unique properties of innovative nanomaterials, providing alternative solutions in diagnostics, prevention and therapeutics. Titanium dioxide nanoparticles (TiO2NPs) have a great spectrum of photocatalytic antibacterial and anticancer applications. The chemical modification of TiO2optimizes its bioactive performance. The aim of this study was the development of silver modified NPs (Ag/TiO2NPs) with anticancer potential. Materials and Methods: Ag/TiO2NPs were prepared through the sol-gel method, were fully characterized and were tested on cultured breast cancer epithelial cells (MCF-7 and MDAMB-231). The MTT colorimetric assay was used to estimate cellular viability. Western blot analysis of protein expression along with a DNA-laddering assay were employed for apoptosis detection. Results and Conclusion: We show that photo-activated Ag/TiO2NPs exhibited significant cytotoxicity on the highly malignant MDA-MB-231 cancer cells, inducing apoptosis, while MCF-7 cells that are characterized by low invasive properties were unaffected under the same conditions.",

keywords = "Anticancer properties, Apoptosis, Cytotoxicity, Nanomedicine, Nanostructured titanium dioxide, Photocatalysis, Silvermodified titanium dioxide, Nanostructures/chemistry, Humans, Nanomedicine/methods, Silver/chemistry, Titanium/pharmacology, Neoplasms/chemically induced",

author = "Nefeli Lagopati and Athanassios Kotsinas and Dimitris Veroutis and Konstantinos Evangelou and Angelos Papaspyropoulos and Michalis Arfanis and Polycarpos Falaras and Kitsiou, {Paraskevi V.} and Ioannis Pateras and Anna Bergonzini and Teresa Frisan and Spyridon Kyriazis and Tsoukleris, {Dimitrios S.} and Tsilibary, {Effie Photini C.} and Maria Gazouli and Pavlatou, {Evangelia A.} and Gorgoulis, {Vassilis G.}",

note = "Funding Information: This study was financially supported by the National Public Investment Program of the Ministry of Development and Investment/General Secretariat for Research and Technology, in the framework of the of the Flagship Initiative to address SARS-CoV-2 (2020ΣΕ01300001). It was also supported by the Welfare Foundation for Social & Cultural Sciences, Athens, Greece (KIKPE); the Hellenic Foundation for Research and Innovation (HFRI) grants no. 775 and 3782 and NKUA-SARG grant 70/3/8916, by the kind donation of H. Pappas and by IKY scholarships program (action “Reinforcement of Postdoctoral Researchers”, (N. Lagopati-scholarship for Postdoctoral Researchers-MIS5001552). Publisher Copyright: {\textcopyright} 2021 International Institute of Anticancer Research. All rights reserved.",

year = "2021",

doi = "10.21873/CGP.20269",

language = "English (US)",

volume = "18",

pages = "425--439",

journal = "Cancer Genomics and Proteomics",

issn = "1109-6535",

publisher = "International Institute of Anticancer Research",

number = "3 Suppl",

}

TY - JOUR

T1 - Biological effect of silver-modified nanostructured titanium dioxide in cancer

AU - Lagopati, Nefeli

AU - Kotsinas, Athanassios

AU - Veroutis, Dimitris

AU - Evangelou, Konstantinos

AU - Papaspyropoulos, Angelos

AU - Arfanis, Michalis

AU - Falaras, Polycarpos

AU - Kitsiou, Paraskevi V.

AU - Pateras, Ioannis

AU - Bergonzini, Anna

AU - Frisan, Teresa

AU - Kyriazis, Spyridon

AU - Tsoukleris, Dimitrios S.

AU - Tsilibary, Effie Photini C.

AU - Gazouli, Maria

AU - Pavlatou, Evangelia A.

AU - Gorgoulis, Vassilis G.

N1 - Funding Information: This study was financially supported by the National Public Investment Program of the Ministry of Development and Investment/General Secretariat for Research and Technology, in the framework of the of the Flagship Initiative to address SARS-CoV-2 (2020ΣΕ01300001). It was also supported by the Welfare Foundation for Social & Cultural Sciences, Athens, Greece (KIKPE); the Hellenic Foundation for Research and Innovation (HFRI) grants no. 775 and 3782 and NKUA-SARG grant 70/3/8916, by the kind donation of H. Pappas and by IKY scholarships program (action “Reinforcement of Postdoctoral Researchers”, (N. Lagopati-scholarship for Postdoctoral Researchers-MIS5001552). Publisher Copyright: © 2021 International Institute of Anticancer Research. All rights reserved.

PY - 2021

Y1 - 2021

N2 - Background/Aim: Nanomedicine is a promising scientific field that exploits the unique properties of innovative nanomaterials, providing alternative solutions in diagnostics, prevention and therapeutics. Titanium dioxide nanoparticles (TiO2NPs) have a great spectrum of photocatalytic antibacterial and anticancer applications. The chemical modification of TiO2optimizes its bioactive performance. The aim of this study was the development of silver modified NPs (Ag/TiO2NPs) with anticancer potential. Materials and Methods: Ag/TiO2NPs were prepared through the sol-gel method, were fully characterized and were tested on cultured breast cancer epithelial cells (MCF-7 and MDAMB-231). The MTT colorimetric assay was used to estimate cellular viability. Western blot analysis of protein expression along with a DNA-laddering assay were employed for apoptosis detection. Results and Conclusion: We show that photo-activated Ag/TiO2NPs exhibited significant cytotoxicity on the highly malignant MDA-MB-231 cancer cells, inducing apoptosis, while MCF-7 cells that are characterized by low invasive properties were unaffected under the same conditions.

AB - Background/Aim: Nanomedicine is a promising scientific field that exploits the unique properties of innovative nanomaterials, providing alternative solutions in diagnostics, prevention and therapeutics. Titanium dioxide nanoparticles (TiO2NPs) have a great spectrum of photocatalytic antibacterial and anticancer applications. The chemical modification of TiO2optimizes its bioactive performance. The aim of this study was the development of silver modified NPs (Ag/TiO2NPs) with anticancer potential. Materials and Methods: Ag/TiO2NPs were prepared through the sol-gel method, were fully characterized and were tested on cultured breast cancer epithelial cells (MCF-7 and MDAMB-231). The MTT colorimetric assay was used to estimate cellular viability. Western blot analysis of protein expression along with a DNA-laddering assay were employed for apoptosis detection. Results and Conclusion: We show that photo-activated Ag/TiO2NPs exhibited significant cytotoxicity on the highly malignant MDA-MB-231 cancer cells, inducing apoptosis, while MCF-7 cells that are characterized by low invasive properties were unaffected under the same conditions.

KW - Anticancer properties

KW - Apoptosis

KW - Cytotoxicity

KW - Nanomedicine

KW - Nanostructured titanium dioxide

KW - Photocatalysis

KW - Silvermodified titanium dioxide

KW - Nanostructures/chemistry

KW - Humans

KW - Nanomedicine/methods

KW - Silver/chemistry

KW - Titanium/pharmacology

KW - Neoplasms/chemically induced

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UR - http://www.scopus.com/inward/citedby.url?scp=85106628567&partnerID=8YFLogxK

U2 - 10.21873/CGP.20269

DO - 10.21873/CGP.20269

M3 - Article

C2 - 33994365

AN - SCOPUS:85106628567

SN - 1109-6535

VL - 18

SP - 425

EP - 439

JO - Cancer Genomics and Proteomics

JF - Cancer Genomics and Proteomics

IS - 3 Suppl

ER -

Biological effect of silver-modified nanostructured titanium dioxide in cancer

Abstract

Bibliographical note

Keywords

PubMed: MeSH publication types

UN SDGs

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