Biological and molecular characterization of a canine hemangiosarcoma-derived cell line

Douglas H. Thamm, Erin B. Dickerson, Nasim Akhtar, Rachel Lewis, Robert Auerbach, Stuart C. Helfand, E. Gregory MacEwen

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Canine hemangiosarcoma (HSA) is a devastating disease. Investigation of novel therapies has been limited by the limited availability of canine HSA-derived cell lines. We report the development of a canine HSA-derived cell line, DEN-HSA, which recapitulates features of angiogenic endothelium. DEN-HSA cells were derived from a spontaneous HSA arising in the kidney of a dog. DEN-HSA displayed surface molecules distinctive of endothelial histogenesis, including factor VIII-related antigen, ICAM-1 and αvβ3 integrin. In vitro, DEN-HSA formed microvascular tube-like structures on Matrigel®, and proliferated in response to a variety of angiogenic growth factors. The cells expressed mRNA and protein specific for bFGF and its receptors, and VEGF and its receptors, among others. DEN-HSA conditioned medium evoked a marked angiogenic response in a murine corneal pocket assay, indicating potent proangiogenic activity of substances secreted by this cell line. This research confirms the DEN-HSA cell line as endothelial in origin, suggests the presence of angiogenic growth factor autocrine loops, and offers the potential to utilize DEN-HSA cells for the study of novel therapies that modulate endothelial proliferation.

Original languageEnglish (US)
Pages (from-to)76-86
Number of pages11
JournalResearch in veterinary science
Volume81
Issue number1
DOIs
StatePublished - Aug 1 2006

Keywords

  • Angiogenesis
  • Angiosarcoma
  • Dog
  • Endothelium

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