Biogas upgrading and biochemical production from gas fermentation: Impact of microbial community and gas composition

Basma Omar; Maie El-Gammal; Reda Abou-Shanab; Ioannis A. Fotidis; Irini Angelidaki; Yifeng Zhang

doi:10.1016/j.biortech.2019.121413

Biogas upgrading and biochemical production from gas fermentation: Impact of microbial community and gas composition

Basma Omar, Maie El-Gammal, Reda Abou-Shanab, Ioannis A. Fotidis, Irini Angelidaki, Yifeng Zhang

Biotechnology Institute

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

The present study proposes a novel alternative method of the current biogas upgrading techniques by converting CO₂ (in the biogas)into valuable chemicals (e.g., volatile fatty acids)using H₂ as energy source and acetogenic mixed culture as biocatalyst. The influence of thermal treatment (90 °C)on the inhibition of the methanogenic archaea and enriching the acetogenic bacteria in different inocula (mesophilic and thermophilic)was initially tested. The most efficient inoculum that achieved the highest performance through the fermentation process was further used to define the optimum H₂/CO₂ gas ratio that secures maximum production yield of chemicals and maximum biogas upgrading efficiency. In addition, 16S rRNA analysis of the microbial community was conducted at the end of the experimental period to target functional microbes. The maximum biogas content (77% (v/v))and acetate yield (72%)were achieved for 2H₂:1CO₂ ratio (v/v), with Moorella sp. 4 as the most dominant thermophilic acetogenic bacterium.

Original language	English (US)
Article number	121413
Journal	Bioresource Technology
Volume	286
DOIs	https://doi.org/10.1016/j.biortech.2019.121413
State	Published - Aug 2019

Bibliographical note

Funding Information:
Hector Garcia was thankful for technical support. Novo Nordisk Foundation (NNF16OC0021568) and Danish Council for Independent Research (DFF-1335-00142) had been corroborative financially this research. The Ministry of Higher Education of Egypt is gratefully acknowledged for PhD channel mission to the first author. The authors are grateful to Dr. Panagiotis Kougias and Dr. Laura Treu for helping in microbiological data analysis.

Publisher Copyright:
© 2019 Elsevier Ltd

Keywords

Acetogens
Acetyl-CoA pathway
Biogas upgrading
Gas fermentation
Microbial community

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2019.121413

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abstract = "The present study proposes a novel alternative method of the current biogas upgrading techniques by converting CO2 (in the biogas)into valuable chemicals (e.g., volatile fatty acids)using H2 as energy source and acetogenic mixed culture as biocatalyst. The influence of thermal treatment (90 °C)on the inhibition of the methanogenic archaea and enriching the acetogenic bacteria in different inocula (mesophilic and thermophilic)was initially tested. The most efficient inoculum that achieved the highest performance through the fermentation process was further used to define the optimum H2/CO2 gas ratio that secures maximum production yield of chemicals and maximum biogas upgrading efficiency. In addition, 16S rRNA analysis of the microbial community was conducted at the end of the experimental period to target functional microbes. The maximum biogas content (77% (v/v))and acetate yield (72%)were achieved for 2H2:1CO2 ratio (v/v), with Moorella sp. 4 as the most dominant thermophilic acetogenic bacterium.",

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note = "Funding Information: Hector Garcia was thankful for technical support. Novo Nordisk Foundation (NNF16OC0021568) and Danish Council for Independent Research (DFF-1335-00142) had been corroborative financially this research. The Ministry of Higher Education of Egypt is gratefully acknowledged for PhD channel mission to the first author. The authors are grateful to Dr. Panagiotis Kougias and Dr. Laura Treu for helping in microbiological data analysis. Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

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AU - Omar, Basma

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AU - Fotidis, Ioannis A.

AU - Angelidaki, Irini

AU - Zhang, Yifeng

N1 - Funding Information: Hector Garcia was thankful for technical support. Novo Nordisk Foundation (NNF16OC0021568) and Danish Council for Independent Research (DFF-1335-00142) had been corroborative financially this research. The Ministry of Higher Education of Egypt is gratefully acknowledged for PhD channel mission to the first author. The authors are grateful to Dr. Panagiotis Kougias and Dr. Laura Treu for helping in microbiological data analysis. Publisher Copyright: © 2019 Elsevier Ltd

PY - 2019/8

Y1 - 2019/8

N2 - The present study proposes a novel alternative method of the current biogas upgrading techniques by converting CO2 (in the biogas)into valuable chemicals (e.g., volatile fatty acids)using H2 as energy source and acetogenic mixed culture as biocatalyst. The influence of thermal treatment (90 °C)on the inhibition of the methanogenic archaea and enriching the acetogenic bacteria in different inocula (mesophilic and thermophilic)was initially tested. The most efficient inoculum that achieved the highest performance through the fermentation process was further used to define the optimum H2/CO2 gas ratio that secures maximum production yield of chemicals and maximum biogas upgrading efficiency. In addition, 16S rRNA analysis of the microbial community was conducted at the end of the experimental period to target functional microbes. The maximum biogas content (77% (v/v))and acetate yield (72%)were achieved for 2H2:1CO2 ratio (v/v), with Moorella sp. 4 as the most dominant thermophilic acetogenic bacterium.

AB - The present study proposes a novel alternative method of the current biogas upgrading techniques by converting CO2 (in the biogas)into valuable chemicals (e.g., volatile fatty acids)using H2 as energy source and acetogenic mixed culture as biocatalyst. The influence of thermal treatment (90 °C)on the inhibition of the methanogenic archaea and enriching the acetogenic bacteria in different inocula (mesophilic and thermophilic)was initially tested. The most efficient inoculum that achieved the highest performance through the fermentation process was further used to define the optimum H2/CO2 gas ratio that secures maximum production yield of chemicals and maximum biogas upgrading efficiency. In addition, 16S rRNA analysis of the microbial community was conducted at the end of the experimental period to target functional microbes. The maximum biogas content (77% (v/v))and acetate yield (72%)were achieved for 2H2:1CO2 ratio (v/v), with Moorella sp. 4 as the most dominant thermophilic acetogenic bacterium.

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Biogas upgrading and biochemical production from gas fermentation: Impact of microbial community and gas composition

Abstract

Bibliographical note

Keywords

UN SDGs

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