Biodegradation of perchloroethylene and chlorophenol co-contamination and toxic effect on activated sludge performance

Hui Li; Ting Ting Shen; Xiao Li Wang; Kuang Fei Lin; Yong Di Liu; Shu Guang Lu; Ji Dong Gu; Ping Wang; Qiang Lu; Xiao Ming Du

doi:10.1016/j.biortech.2013.02.050

Biodegradation of perchloroethylene and chlorophenol co-contamination and toxic effect on activated sludge performance

Hui Li, Ting Ting Shen, Xiao Li Wang, Kuang Fei Lin, Yong Di Liu, Shu Guang Lu, Ji Dong Gu, Ping Wang, Qiang Lu, Xiao Ming Du

Bioproducts and Biosystems Engineering

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

This study investigated the effects of PCE and 2-CP co-contamination on growth of microbial community in terms of enzymatic activity and microbial diversity in activated sludge. Results showed that the activities of three key enzymes (dehydrogenase, phosphatase and urease) decreased significantly when PCE (in the range of 5-150. mg/L) was mixed with 2-CP (in the range of 25-150. mg/L). Especially, activity of dehydrogenase decreased by more than 93% as the concentration of PCE and 2-CP both reached 150. mg/L. PCR-DGGE revealed that short-term exposure with PCE and 2-CP did not lead to shift in the microbial community structure, while clone library demonstrated a significant change in the microbial diversity after long-term exposure. As the population of Alphaproteobacteria and Gamaproteobacteria decreased, with Actinobacteria eventually disappeared, species including Firmicutes, Bacteroidetes and Synergistetes became dominating groups. This study demonstrated that co-contamination with PCE and 2-CP affected the performance of activated sludge in a significant way.

Original language	English (US)
Pages (from-to)	286-293
Number of pages	8
Journal	Bioresource Technology
Volume	137
DOIs	https://doi.org/10.1016/j.biortech.2013.02.050
State	Published - Jun 2013

Bibliographical note

Funding Information:
This research was financially supported by the National Natural Science Foundation of China ( 41003031 , 41273109 ), the Shanghai Rising-Star Program ( 12QA1400800 ), the Specialized Research Fund for the Doctoral Program of Higher Education ( 20110074130002 ), the National Environmental Protection Public Welfare Science and Technology Research Program of China ( 200909089 , 2010467032 , 201109013 ), the Key Science Foundation of Shanghai Environmental Protection Bureau (Huhuanke2012-07), the Fundamental Research Funds for the Central Universities (WB1014004,WB1314002), the National Science Foundation for Postdoctoral Scientists of China ( 2012M510811 ). We also would like to thank the anonymous referees for their helpful comments on this paper.

Keywords

Activated sludge
Co-contamination
Enzyme activity
Microbial community diversity

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2013.02.050

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@article{bf3014b68cf74c85963be195287a0fa2,

title = "Biodegradation of perchloroethylene and chlorophenol co-contamination and toxic effect on activated sludge performance",

abstract = "This study investigated the effects of PCE and 2-CP co-contamination on growth of microbial community in terms of enzymatic activity and microbial diversity in activated sludge. Results showed that the activities of three key enzymes (dehydrogenase, phosphatase and urease) decreased significantly when PCE (in the range of 5-150. mg/L) was mixed with 2-CP (in the range of 25-150. mg/L). Especially, activity of dehydrogenase decreased by more than 93% as the concentration of PCE and 2-CP both reached 150. mg/L. PCR-DGGE revealed that short-term exposure with PCE and 2-CP did not lead to shift in the microbial community structure, while clone library demonstrated a significant change in the microbial diversity after long-term exposure. As the population of Alphaproteobacteria and Gamaproteobacteria decreased, with Actinobacteria eventually disappeared, species including Firmicutes, Bacteroidetes and Synergistetes became dominating groups. This study demonstrated that co-contamination with PCE and 2-CP affected the performance of activated sludge in a significant way.",

keywords = "Activated sludge, Co-contamination, Enzyme activity, Microbial community diversity",

author = "Hui Li and Shen, {Ting Ting} and Wang, {Xiao Li} and Lin, {Kuang Fei} and Liu, {Yong Di} and Lu, {Shu Guang} and Gu, {Ji Dong} and Ping Wang and Qiang Lu and Du, {Xiao Ming}",

note = "Funding Information: This research was financially supported by the National Natural Science Foundation of China ( 41003031 , 41273109 ), the Shanghai Rising-Star Program ( 12QA1400800 ), the Specialized Research Fund for the Doctoral Program of Higher Education ( 20110074130002 ), the National Environmental Protection Public Welfare Science and Technology Research Program of China ( 200909089 , 2010467032 , 201109013 ), the Key Science Foundation of Shanghai Environmental Protection Bureau (Huhuanke2012-07), the Fundamental Research Funds for the Central Universities (WB1014004,WB1314002), the National Science Foundation for Postdoctoral Scientists of China ( 2012M510811 ). We also would like to thank the anonymous referees for their helpful comments on this paper.",

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T1 - Biodegradation of perchloroethylene and chlorophenol co-contamination and toxic effect on activated sludge performance

AU - Li, Hui

AU - Shen, Ting Ting

AU - Wang, Xiao Li

AU - Lin, Kuang Fei

AU - Liu, Yong Di

AU - Lu, Shu Guang

AU - Gu, Ji Dong

AU - Wang, Ping

AU - Lu, Qiang

AU - Du, Xiao Ming

N1 - Funding Information: This research was financially supported by the National Natural Science Foundation of China ( 41003031 , 41273109 ), the Shanghai Rising-Star Program ( 12QA1400800 ), the Specialized Research Fund for the Doctoral Program of Higher Education ( 20110074130002 ), the National Environmental Protection Public Welfare Science and Technology Research Program of China ( 200909089 , 2010467032 , 201109013 ), the Key Science Foundation of Shanghai Environmental Protection Bureau (Huhuanke2012-07), the Fundamental Research Funds for the Central Universities (WB1014004,WB1314002), the National Science Foundation for Postdoctoral Scientists of China ( 2012M510811 ). We also would like to thank the anonymous referees for their helpful comments on this paper.

PY - 2013/6

Y1 - 2013/6

N2 - This study investigated the effects of PCE and 2-CP co-contamination on growth of microbial community in terms of enzymatic activity and microbial diversity in activated sludge. Results showed that the activities of three key enzymes (dehydrogenase, phosphatase and urease) decreased significantly when PCE (in the range of 5-150. mg/L) was mixed with 2-CP (in the range of 25-150. mg/L). Especially, activity of dehydrogenase decreased by more than 93% as the concentration of PCE and 2-CP both reached 150. mg/L. PCR-DGGE revealed that short-term exposure with PCE and 2-CP did not lead to shift in the microbial community structure, while clone library demonstrated a significant change in the microbial diversity after long-term exposure. As the population of Alphaproteobacteria and Gamaproteobacteria decreased, with Actinobacteria eventually disappeared, species including Firmicutes, Bacteroidetes and Synergistetes became dominating groups. This study demonstrated that co-contamination with PCE and 2-CP affected the performance of activated sludge in a significant way.

AB - This study investigated the effects of PCE and 2-CP co-contamination on growth of microbial community in terms of enzymatic activity and microbial diversity in activated sludge. Results showed that the activities of three key enzymes (dehydrogenase, phosphatase and urease) decreased significantly when PCE (in the range of 5-150. mg/L) was mixed with 2-CP (in the range of 25-150. mg/L). Especially, activity of dehydrogenase decreased by more than 93% as the concentration of PCE and 2-CP both reached 150. mg/L. PCR-DGGE revealed that short-term exposure with PCE and 2-CP did not lead to shift in the microbial community structure, while clone library demonstrated a significant change in the microbial diversity after long-term exposure. As the population of Alphaproteobacteria and Gamaproteobacteria decreased, with Actinobacteria eventually disappeared, species including Firmicutes, Bacteroidetes and Synergistetes became dominating groups. This study demonstrated that co-contamination with PCE and 2-CP affected the performance of activated sludge in a significant way.

KW - Activated sludge

KW - Co-contamination

KW - Enzyme activity

KW - Microbial community diversity

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Biodegradation of perchloroethylene and chlorophenol co-contamination and toxic effect on activated sludge performance

Abstract

Bibliographical note

Keywords

UN SDGs

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