Biodecolorization and biodegradation of reactive Levafix Blue E-RA granulate dye by the white rot fungus Irpex lacteus

Duraisamy Kalpana, Natarajan Velmurugan, Jae Hong Shim, Byung Taek Oh, Kalaiselvi Senthil, Yang Soo Lee

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

The treatment of effluents from textile industry with microorganisms, especially bacteria and fungi, has recently gained attention. The present study was conducted using white rot fungi Irpex lacteus, Trametes hirsuta, Trametes sp., and Lentinula edodes for the decolorization of reactive textile Levafix Blue E-RA granulate dye. I. lacteus resulted in the best decolorization and degradation of the dye within four days. Therefore, more detailed studies were carried out using I. lacteus. The decolorization was evaluated at various concentration, pH values, and temperatures. The activities of laccase, manganese peroxidase, and lignin peroxidase enzymes were estimated to reveal the roles of enzymes in decolorization. The colorless nature of the fungal cells revealed that decolorization occurred through degradation, and confirmed by analysis of the metabolites by UV-visible spectroscopy and High Performance Liquid Chromatography after decolorization. The metabolites were identified by Gas Chromatography-Mass Spectrometry, and functional group analysis was performed by Fourier Transform Infrared Spectroscopy. The degraded dye metabolites were assessed for phytotoxicity using Vigna radiata and Brassica juncea, which demonstrated nontoxic nature of the metabolites formed after degradation of dye.

Original languageEnglish (US)
Pages (from-to)142-149
Number of pages8
JournalJournal of Environmental Management
Volume111
DOIs
StatePublished - Nov 30 2012

Keywords

  • Biodegradation
  • Decolorization
  • Irpex lacteus
  • Laccase
  • Levafix Blue E-RA granulate
  • Phytotoxicity

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