Bioconversion of p-coumaric acid to p-hydroxystyrene using phenolic acid decarboxylase from B. amyloliquefaciens in biphasic reaction system

Da Hye Jung, Wonji Choi, Kwon Young Choi, Eunok Jung, Hyungdon Yun, Romas J. Kazlauskas, Byung Gee Kim

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Phenolic acid decarboxylase (PAD) catalyzes the non-oxidative decarboxylation of p-coumaric acid (pCA) to p-hydroxystyrene (pHS). PAD from Bacillus amyloliquefaciens (BAPAD), which showed k cat/K m value for pCA (9.3 × 103 mM-1 s-1), was found as the most active one using the "Subgrouping Automata" program and by comparing enzyme activity. However, the production of pHS of recombinant Escherichia coli harboring BAPAD showed only a 22.7 % conversion yield due to product inhibition. Based on the partition coefficient of pHS and biocompatibility of the cell, 1-octanol was selected for the biphasic reaction. The conversion yield increased up to 98.0 % and 0.83 g/h/g DCW productivity was achieved at 100 mM pCA using equal volume of 1-octanol as an organic solvent. In the optimized biphasic reactor, using a three volume ratio of 1-octanol to phosphate buffer phase (50 mM, pH 7.0), the recombinant E. coli produced pHS with a 88.7 % conversion yield and 1.34 g/h/g DCW productivity at 300 mM pCA.

Original languageEnglish (US)
Pages (from-to)1501-1511
Number of pages11
JournalApplied Microbiology and Biotechnology
Volume97
Issue number4
DOIs
StatePublished - Feb 2013

Bibliographical note

Funding Information:
Acknowledgments This research was supported by World Class University program through the National Research Foundation of Korea grant funded by the Ministry of Education, Science and Technology (R322009000102130).

Keywords

  • 1-Octanol
  • Bioconversion
  • Biphasic reaction
  • Phenolic acid decarboxylase
  • p-Coumaric acid
  • p-Hydroxystyrene

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