Bioconjugation of gold nanoparticles through the oxidative coupling of ortho-aminophenols and anilines

Stacy L Capehart, Adel M. Elsohly, Allie C. Obermeyer, Matthew B. Francis

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

While there are a number of methods for attaching gold nanoparticles (AuNPs) to biomolecules, the existing strategies suffer from nonspecific AuNP adsorption, reagents that are unstable in aqueous solutions, and/or long reaction times. To improve upon existing AuNP bioconjugation strategies, we have adapted a recently reported potassium ferricyanide-mediated oxidative coupling reaction for the attachment of aniline-functionalized AuNPs to o-aminophenol-containing oligonucleotides, peptides, and proteins. The aniline-AuNPs are stable in aqueous solutions, show little-to-no nonspecific adsorption with biomolecules, and react rapidly (30 min) with o-aminophenols under mild conditions (pH 6.5, 1 mM oxidant).

Original languageEnglish (US)
Pages (from-to)1888-1892
Number of pages5
JournalBioconjugate Chemistry
Volume25
Issue number10
DOIs
StatePublished - Oct 15 2014

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