Abstract
We develop a simple and biocompatible method of patterning proteins on a wettability gradient surface by thermo-transfer printing. The wettability gradient is produced on a poly(dimethylsiloxane) (PDMS)-modified glass substrate through the temperature gradient during thermo-transfer printing. The water contact angle on the PDMS-modified surface is found to gradually increase along the direction of the temperature gradient from a low to a high temperature region. Based on the wettability gradient, the gradual change in the adsorption and immobilization of proteins (cholera toxin B subunit) is achieved in a microfluidic cell with the PDMS-modified surface.
Original language | English (US) |
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Pages (from-to) | 6069-6071 |
Number of pages | 3 |
Journal | Journal of Nanoscience and Nanotechnology |
Volume | 14 |
Issue number | 8 |
DOIs | |
State | Published - Aug 2014 |
Keywords
- PDMS transfer
- Protein array
- Protein binding
- Wettability gradient