Biochemical study of protease inhibitors in normal chinchilla middle ear

Yukiyoshi Hamaguchi, Steven K. Juhn, Yasuo Sakakura

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Protein concentration and inhibitory capacity of both α1-antitrypsin (α1-AT) and α2-macroglobulin (α2-M) were measured in plasma and middle ear bulla (MEB) washings of chinchillas by use of specific antisera against chinchilla α1-AT and α2-M. Low molecular weight (LMW) trypsin inhibitor also was analyzed in MEB washings. Chinchilla α2-M showed a common antigenicity with human α2-M. The mean value of α1-AT in chinchilla plasma was 412.0 ± 87.8 and that of α2-M was 435.0 ± 117.1 mg/dL. There was a significant relationship between α-AT level and antitryptic activity, and between α2-M level and trypsin binding activity in plasma. The majority of α1-AT and α2-M in plasma is present as free inhibitors unsaturated with proteases. The MEB washings had significant antitryptic activity, which is attributed to both α1-AT and LMW trypsin inhibitors. Inhibitory functions of α1-AT and LMW trypsin inhibitors appear to play an important role in the defense of the normal middle ear mucosa.

Original languageEnglish (US)
Pages (from-to)472-478
Number of pages7
JournalAnnals of Otology, Rhinology & Laryngology
Issue number6
StatePublished - Jun 1989


  • antitryptic activity
  • low molecular weight trypsin inhibitor
  • middle ear bulla washing
  • trypsin binding activity
  • α1-antitrypsin
  • α2-macroglobulin


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