Biochar affects community composition of nitrous oxide reducers in a field experiment

Hans Martin Krause; Roman Hüppi; Jens Leifeld; Mohamed El-Hadidi; Johannes Harter; Andreas Kappler; Martin Hartmann; Sebastian Behrens; Paul Mäder; Andreas Gattinger

doi:10.1016/j.soilbio.2018.01.018

Biochar affects community composition of nitrous oxide reducers in a field experiment

Hans Martin Krause, Roman Hüppi, Jens Leifeld, Mohamed El-Hadidi, Johannes Harter, Andreas Kappler, Martin Hartmann, Sebastian Behrens, Paul Mäder, Andreas Gattinger

Civil, Environmental, and Geo- Engineering

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

N₂O is a major greenhouse gas and the majority of anthropogenic N₂O emissions originate from agriculturally managed soils. Therefore, developing N₂O mitigation strategies is a key challenge for the agricultural sector and biochar soil treatment is one reported option. Biochar's capacity to increase soil pH and to foster activity of specialized N₂O reducers has been proposed as possible mechanisms for N₂O mitigation. An experiment was undertaken to investigate whether changes in the community composition of N₂O reducers was observed under field conditions after biochar application. The study objective was to assess the abundance and taxonomic composition of the functional marker genes nosZ and nosZ –II across a vegetation period of Zea mays L. after biochar or lime addition compared to an untreated control. After fertilization, biochar amendment resulted in a significant increase of nosZ gene copy numbers compared to the control and the lime treatment. Simultaneously a shift in community composition of nosZ-II bearing bacteria was observed in the biochar treatment that went beyond the sole liming effect. This study broadens our understanding of the functional impact of biochar on N₂O emissions and emphasizes the possibility to shape the functioning of the N₂O reducing microbial community through the addition of biochar at a field scale.

Original language	English (US)
Pages (from-to)	143-151
Number of pages	9
Journal	Soil Biology and Biochemistry
Volume	119
DOIs	https://doi.org/10.1016/j.soilbio.2018.01.018
State	Published - Apr 2018

Bibliographical note

Funding Information:
We would like to thank the SNF for the financial support in frame of the National Research Program 68 “Soil as a Resource” (Grant Nr. 406840_143137 ). We also acknowledge the contribution of scientists at the McGill University and Génome Québec Innovation Center in Montréal, Canada, for the paired-end sequencing on Illumina MiSeq. Furthermore we would like to thank Simon Moakes for English correction. The authors declare to have no conflict of interest.

Funding Information:
We would like to thank the SNF for the financial support in frame of the National Research Program 68 “Soil as a Resource” (Grant Nr. 406840_143137). We also acknowledge the contribution of scientists at the McGill University and Génome Québec Innovation Center in Montréal, Canada, for the paired-end sequencing on Illumina MiSeq. Furthermore we would like to thank Simon Moakes for English correction. The authors declare to have no conflict of interest.

Publisher Copyright:
© 2018 Elsevier Ltd

Keywords

Biochar
Community composition
Field trial
Functional diversity
NO reduction
nosZ

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Publisher link

10.1016/j.soilbio.2018.01.018

Find It

Find It at U of M Libraries

Cite this

@article{330fbebfc6894af5a338f6bbdf6872c8,

title = "Biochar affects community composition of nitrous oxide reducers in a field experiment",

abstract = "N2O is a major greenhouse gas and the majority of anthropogenic N2O emissions originate from agriculturally managed soils. Therefore, developing N2O mitigation strategies is a key challenge for the agricultural sector and biochar soil treatment is one reported option. Biochar's capacity to increase soil pH and to foster activity of specialized N2O reducers has been proposed as possible mechanisms for N2O mitigation. An experiment was undertaken to investigate whether changes in the community composition of N2O reducers was observed under field conditions after biochar application. The study objective was to assess the abundance and taxonomic composition of the functional marker genes nosZ and nosZ –II across a vegetation period of Zea mays L. after biochar or lime addition compared to an untreated control. After fertilization, biochar amendment resulted in a significant increase of nosZ gene copy numbers compared to the control and the lime treatment. Simultaneously a shift in community composition of nosZ-II bearing bacteria was observed in the biochar treatment that went beyond the sole liming effect. This study broadens our understanding of the functional impact of biochar on N2O emissions and emphasizes the possibility to shape the functioning of the N2O reducing microbial community through the addition of biochar at a field scale.",

keywords = "Biochar, Community composition, Field trial, Functional diversity, NO reduction, nosZ",

author = "Krause, {Hans Martin} and Roman H{\"u}ppi and Jens Leifeld and Mohamed El-Hadidi and Johannes Harter and Andreas Kappler and Martin Hartmann and Sebastian Behrens and Paul M{\"a}der and Andreas Gattinger",

note = "Funding Information: We would like to thank the SNF for the financial support in frame of the National Research Program 68 “Soil as a Resource” (Grant Nr. 406840_143137 ). We also acknowledge the contribution of scientists at the McGill University and G{\'e}nome Qu{\'e}bec Innovation Center in Montr{\'e}al, Canada, for the paired-end sequencing on Illumina MiSeq. Furthermore we would like to thank Simon Moakes for English correction. The authors declare to have no conflict of interest. Funding Information: We would like to thank the SNF for the financial support in frame of the National Research Program 68 “Soil as a Resource” (Grant Nr. 406840_143137). We also acknowledge the contribution of scientists at the McGill University and G{\'e}nome Qu{\'e}bec Innovation Center in Montr{\'e}al, Canada, for the paired-end sequencing on Illumina MiSeq. Furthermore we would like to thank Simon Moakes for English correction. The authors declare to have no conflict of interest. Publisher Copyright: {\textcopyright} 2018 Elsevier Ltd",

year = "2018",

month = apr,

doi = "10.1016/j.soilbio.2018.01.018",

language = "English (US)",

volume = "119",

pages = "143--151",

journal = "Soil Biology and Biochemistry",

issn = "0038-0717",

publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Biochar affects community composition of nitrous oxide reducers in a field experiment

AU - Krause, Hans Martin

AU - Hüppi, Roman

AU - Leifeld, Jens

AU - El-Hadidi, Mohamed

AU - Harter, Johannes

AU - Kappler, Andreas

AU - Hartmann, Martin

AU - Behrens, Sebastian

AU - Mäder, Paul

AU - Gattinger, Andreas

N1 - Funding Information: We would like to thank the SNF for the financial support in frame of the National Research Program 68 “Soil as a Resource” (Grant Nr. 406840_143137 ). We also acknowledge the contribution of scientists at the McGill University and Génome Québec Innovation Center in Montréal, Canada, for the paired-end sequencing on Illumina MiSeq. Furthermore we would like to thank Simon Moakes for English correction. The authors declare to have no conflict of interest. Funding Information: We would like to thank the SNF for the financial support in frame of the National Research Program 68 “Soil as a Resource” (Grant Nr. 406840_143137). We also acknowledge the contribution of scientists at the McGill University and Génome Québec Innovation Center in Montréal, Canada, for the paired-end sequencing on Illumina MiSeq. Furthermore we would like to thank Simon Moakes for English correction. The authors declare to have no conflict of interest. Publisher Copyright: © 2018 Elsevier Ltd

PY - 2018/4

Y1 - 2018/4

N2 - N2O is a major greenhouse gas and the majority of anthropogenic N2O emissions originate from agriculturally managed soils. Therefore, developing N2O mitigation strategies is a key challenge for the agricultural sector and biochar soil treatment is one reported option. Biochar's capacity to increase soil pH and to foster activity of specialized N2O reducers has been proposed as possible mechanisms for N2O mitigation. An experiment was undertaken to investigate whether changes in the community composition of N2O reducers was observed under field conditions after biochar application. The study objective was to assess the abundance and taxonomic composition of the functional marker genes nosZ and nosZ –II across a vegetation period of Zea mays L. after biochar or lime addition compared to an untreated control. After fertilization, biochar amendment resulted in a significant increase of nosZ gene copy numbers compared to the control and the lime treatment. Simultaneously a shift in community composition of nosZ-II bearing bacteria was observed in the biochar treatment that went beyond the sole liming effect. This study broadens our understanding of the functional impact of biochar on N2O emissions and emphasizes the possibility to shape the functioning of the N2O reducing microbial community through the addition of biochar at a field scale.

AB - N2O is a major greenhouse gas and the majority of anthropogenic N2O emissions originate from agriculturally managed soils. Therefore, developing N2O mitigation strategies is a key challenge for the agricultural sector and biochar soil treatment is one reported option. Biochar's capacity to increase soil pH and to foster activity of specialized N2O reducers has been proposed as possible mechanisms for N2O mitigation. An experiment was undertaken to investigate whether changes in the community composition of N2O reducers was observed under field conditions after biochar application. The study objective was to assess the abundance and taxonomic composition of the functional marker genes nosZ and nosZ –II across a vegetation period of Zea mays L. after biochar or lime addition compared to an untreated control. After fertilization, biochar amendment resulted in a significant increase of nosZ gene copy numbers compared to the control and the lime treatment. Simultaneously a shift in community composition of nosZ-II bearing bacteria was observed in the biochar treatment that went beyond the sole liming effect. This study broadens our understanding of the functional impact of biochar on N2O emissions and emphasizes the possibility to shape the functioning of the N2O reducing microbial community through the addition of biochar at a field scale.

KW - Biochar

KW - Community composition

KW - Field trial

KW - Functional diversity

KW - NO reduction

KW - nosZ

UR - http://www.scopus.com/inward/record.url?scp=85041463777&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85041463777&partnerID=8YFLogxK

U2 - 10.1016/j.soilbio.2018.01.018

DO - 10.1016/j.soilbio.2018.01.018

M3 - Article

AN - SCOPUS:85041463777

SN - 0038-0717

VL - 119

SP - 143

EP - 151

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

ER -

Biochar affects community composition of nitrous oxide reducers in a field experiment

Abstract

Bibliographical note

Keywords

UN SDGs

Publisher link

Find It

Other files and links

Fingerprint

Cite this