TY - JOUR
T1 - Bioanalysis of eukaryotic organelles
AU - Satori, Chad P.
AU - Henderson, Michelle M.
AU - Krautkramer, Elyse A.
AU - Kostal, Vratislav
AU - Distefano, Mark D
AU - Arriaga, Edgar
PY - 2013/4/10
Y1 - 2013/4/10
N2 - The role that organelle analysis has played in understanding biology is studied. Organelle analysis enables a more specific description of the molecular, biochemical, and physiological processes associated with diseases, embryonic development, tissue differentiation, organism aging, disease treatments, and organism response to pathogens. Confocal microscopy has become a routine tool for investigating subcellular organization, organelle networks, and organelle dynamics in cellular and tissue samples. Most organelles have a dynamic, three-dimensional (3D) organization inside the cell, which is tightly connected to their physiological functions. Due to this, a single 2D image inherently limits the information acquired about the distribution of a particular property within the organelle. The combination of subcellular fractionation with 'omic' technologies has become a powerful resource to characterize and catalogue the various subcellular environments in a cell.
AB - The role that organelle analysis has played in understanding biology is studied. Organelle analysis enables a more specific description of the molecular, biochemical, and physiological processes associated with diseases, embryonic development, tissue differentiation, organism aging, disease treatments, and organism response to pathogens. Confocal microscopy has become a routine tool for investigating subcellular organization, organelle networks, and organelle dynamics in cellular and tissue samples. Most organelles have a dynamic, three-dimensional (3D) organization inside the cell, which is tightly connected to their physiological functions. Due to this, a single 2D image inherently limits the information acquired about the distribution of a particular property within the organelle. The combination of subcellular fractionation with 'omic' technologies has become a powerful resource to characterize and catalogue the various subcellular environments in a cell.
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U2 - 10.1021/cr300354g
DO - 10.1021/cr300354g
M3 - Review article
C2 - 23570618
AN - SCOPUS:84876127456
SN - 0009-2665
VL - 113
SP - 2733
EP - 2811
JO - Chemical Reviews
JF - Chemical Reviews
IS - 4
ER -