Binding of an X-specific condensin correlates with a reduction in active histone modifications at gene regulatory elements

Lena Annika Street, Ana Karina Morao, Lara Heermans Winterkorn, Chen Yu Jiao, Sarah Elizabeth Albritton, Mohammed Sadic, Maxwell L Kramer, Sevinç Ercan

Research output: Contribution to journalArticle

Abstract

Condensins are evolutionarily conserved protein complexes that are required for chromosome segregation during cell division and genome organization during interphase. In Caenorhabditis elegans, a specialized condensin, which forms the core of the dosage compensation complex (DCC), binds to and represses X chromosome transcription. Here, we analyzed DCC localization and the effect of DCC depletion on histone modifications, transcription factor binding, and gene expression using chromatin immunoprecipitation sequencing and mRNA sequencing. Across the X, the DCC accumulates at accessible gene regulatory sites in active chromatin and not heterochromatin. The DCC is required for reducing the levels of activating histone modifications, including H3K4me3 and H3K27ac, but not repressive modification H3K9me3. In X-to-autosome fusion chromosomes, DCC spreading into the autosomal sequences locally reduces gene expression, thus establishing a direct link between DCC binding and repression. Together, our results indicate that DCC-mediated transcription repression is associated with a reduction in the activity of X chromosomal gene regulatory elements.

Original languageEnglish (US)
Pages (from-to)729-742
Number of pages14
JournalGenetics
Volume212
Issue number3
DOIs
StatePublished - Jul 1 2019

Fingerprint

Histone Code
Regulator Genes
Gene Expression
Chromosome Segregation
Heterochromatin
Chromatin Immunoprecipitation
Interphase
Caenorhabditis elegans
Dosage Forms
X Chromosome
Cell Division
Chromatin
Catalytic Domain
Transcription Factors
Chromosomes
Genome
Messenger RNA
Proteins
condensin complexes

Keywords

  • C. elegans
  • Chromatin
  • Condensin
  • Dosage compensation
  • Gene expression
  • Gene regulation
  • Histone modifications
  • Transcription
  • X chromosome

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural

Cite this

Street, L. A., Morao, A. K., Winterkorn, L. H., Jiao, C. Y., Albritton, S. E., Sadic, M., ... Ercan, S. (2019). Binding of an X-specific condensin correlates with a reduction in active histone modifications at gene regulatory elements. Genetics, 212(3), 729-742. https://doi.org/10.1534/genetics.119.302254

Binding of an X-specific condensin correlates with a reduction in active histone modifications at gene regulatory elements. / Street, Lena Annika; Morao, Ana Karina; Winterkorn, Lara Heermans; Jiao, Chen Yu; Albritton, Sarah Elizabeth; Sadic, Mohammed; Kramer, Maxwell L; Ercan, Sevinç.

In: Genetics, Vol. 212, No. 3, 01.07.2019, p. 729-742.

Research output: Contribution to journalArticle

Street, LA, Morao, AK, Winterkorn, LH, Jiao, CY, Albritton, SE, Sadic, M, Kramer, ML & Ercan, S 2019, 'Binding of an X-specific condensin correlates with a reduction in active histone modifications at gene regulatory elements', Genetics, vol. 212, no. 3, pp. 729-742. https://doi.org/10.1534/genetics.119.302254
Street, Lena Annika ; Morao, Ana Karina ; Winterkorn, Lara Heermans ; Jiao, Chen Yu ; Albritton, Sarah Elizabeth ; Sadic, Mohammed ; Kramer, Maxwell L ; Ercan, Sevinç. / Binding of an X-specific condensin correlates with a reduction in active histone modifications at gene regulatory elements. In: Genetics. 2019 ; Vol. 212, No. 3. pp. 729-742.
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