Biliary excretion of exogenous hematin in rats

Z. J. Petryka, C. A. Pierach, A. Smith, M. N. Goertz, P. S. Edwards

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8 Scopus citations


Rats with bile fistula were injected intravenously with single doses of hematin (5,10,20,30, and 40 mg/kg body weight). Bile samples were collected every 30 min. for 4 hours, and at longer time intervals thereafter. The concentration of hematin in the bile was measured spectrophotometrically at 590 nm. The maximal hematin concentration in the bile (0.29 mg/ml for 40 mg/kg) observed 1.25 hours (average from 15 rats; range 0.5 - 1.5 hours) after hematin administration occured progressively later for lower doses of hematin, and for 5 mg/kg was observed at 3.14 hours (average for 4 rats; range 2-4 hours). The total hematin excreted within 4 hours was 8.0% of injected dose for 40 mg/kg and is dose dependent.

Original languageEnglish (US)
Pages (from-to)1015-1020
Number of pages6
JournalLife Sciences
Issue number7
StatePublished - Oct 1 1977

Bibliographical note

Funding Information:
University of Minnesota Medical Research Unit Northwestern Hospital, Minneapolis, MN 55407 *Scripps Clinic and Research Foundation, La Jolla, CA 92037


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