TY - JOUR
T1 - Bifunctional Peptide-Conjugated Gold Nanoparticles for Precise and Efficient Nucleus-Targeting Bioimaging in Live Cells
AU - Gao, Yingying
AU - Liu, Yueling
AU - Yan, Rui
AU - Zhou, Jinfeng
AU - Dong, Hao
AU - Hua, Xin
AU - Wang, Ping
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (Grant 21804043), Shanghai Sailing Program (Grant 18YF1405800), and the Fundamental Research Funds for the Central Universities (Grant 222201814034).
PY - 2020/10/6
Y1 - 2020/10/6
N2 - Real-time in situ imaging of organelles is increasingly important in modern biomedical analysis and diseases diagnosis. To realize this goal, organelle-targeting nanoparticles as one of the most commonly used technologies in subcellular sensing and imaging has attracted a lot of interest. The biocompatibility, specificity, and binding efficiency are especially critical for efficient organelle-targeting bioimaging. Gold nanoparticles (AuNPs) fabricated with bifunctional peptides constructed with both Au-binding affinity and nucleus-targeting ability were designed and examined for efficient nucleus-targeting bioimaging. Such a design is expected to achieve an oriented assembling of peptides by the medium of the Au-binding peptides specifically assembled on the surface of AuNPs, with the nucleus-targeting end open for accessibility. The bifunctional peptides showed strong binding affinity toward AuNPs and led to a binding capability ∼1.5 times higher than that of the bare nucleus-targeting peptides, ensuring good surface coverage of the nanoparticles for enhanced nucleus-targeting ability. Such fabricated AuNPs demonstrated over 90% cell viability after incubation for 24 h with HepG2 cells, which were highly biocompatible. Precise and efficient bioimaging of the nucleus was achieved for HepG2 cells by using the fabricated AuNPs as observed with a confocal laser scanning microscope, a dark-field/fluorescence microscope, and a transmission electron microscope. The high surface coverage and oriented binding pattern appeared to be a promising strategy for construction of organelle-targeting agencies.
AB - Real-time in situ imaging of organelles is increasingly important in modern biomedical analysis and diseases diagnosis. To realize this goal, organelle-targeting nanoparticles as one of the most commonly used technologies in subcellular sensing and imaging has attracted a lot of interest. The biocompatibility, specificity, and binding efficiency are especially critical for efficient organelle-targeting bioimaging. Gold nanoparticles (AuNPs) fabricated with bifunctional peptides constructed with both Au-binding affinity and nucleus-targeting ability were designed and examined for efficient nucleus-targeting bioimaging. Such a design is expected to achieve an oriented assembling of peptides by the medium of the Au-binding peptides specifically assembled on the surface of AuNPs, with the nucleus-targeting end open for accessibility. The bifunctional peptides showed strong binding affinity toward AuNPs and led to a binding capability ∼1.5 times higher than that of the bare nucleus-targeting peptides, ensuring good surface coverage of the nanoparticles for enhanced nucleus-targeting ability. Such fabricated AuNPs demonstrated over 90% cell viability after incubation for 24 h with HepG2 cells, which were highly biocompatible. Precise and efficient bioimaging of the nucleus was achieved for HepG2 cells by using the fabricated AuNPs as observed with a confocal laser scanning microscope, a dark-field/fluorescence microscope, and a transmission electron microscope. The high surface coverage and oriented binding pattern appeared to be a promising strategy for construction of organelle-targeting agencies.
UR - http://www.scopus.com/inward/record.url?scp=85092023647&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85092023647&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.0c03476
DO - 10.1021/acs.analchem.0c03476
M3 - Article
C2 - 32940455
AN - SCOPUS:85092023647
VL - 92
SP - 13595
EP - 13603
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 19
ER -