BiBAC Modification and Stable Transfer into Maize (Zea mays) Hi-II Immature Embryos via Agrobacterium-Mediated Transformation

Jon P. Cody; Nathaniel D Graham; James A. Birchler

doi:10.1002/cppb.20061

BiBAC Modification and Stable Transfer into Maize (Zea mays) Hi-II Immature Embryos via Agrobacterium-Mediated Transformation

Jon P. Cody, Nathaniel D Graham, James A. Birchler

Genetics, Cell Biology and Development (CBS)

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Binary Bacterial Artificial Chromosomes (BiBAC) are large insert cloning vectors that contain the necessary features required for Agrobacterium-mediated transformation. However, the large size of BiBACs and low-copy number in Escherichia coli (DH10B) and Agrobacterium tumefaciens make cloning experiments more difficult than other available binary vector systems. Therefore, a protocol that outlines preparation, modification, and transformation of high-molecular weight (HMW) constructs is advantageous for researchers looking to use BiBACs in plant genomics research. This unit does not cover the cloning of HMW DNA into BiBAC vectors. Researchers looking to clone HMW DNA into BiBACs can refer to Zhang et al. (2012; doi: 10.1038/nprot.2011.456).

Original language	English (US)
Pages (from-to)	350-369
Number of pages	20
Journal	Current protocols in plant biology
Volume	2
Issue number	4
DOIs	https://doi.org/10.1002/cppb.20061
State	Published - Dec 2017

Bibliographical note

Funding Information:
Research on this topic was supported by grant IOS‐1339198 from the National Science Foundation.

Publisher Copyright:
© 2017 John Wiley & Sons, Inc.

Keywords

Agrobacterium tumefaciens
BiBAC
genetic engineering
transgenes

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10.1002/cppb.20061

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title = "BiBAC Modification and Stable Transfer into Maize (Zea mays) Hi-II Immature Embryos via Agrobacterium-Mediated Transformation",

abstract = "Binary Bacterial Artificial Chromosomes (BiBAC) are large insert cloning vectors that contain the necessary features required for Agrobacterium-mediated transformation. However, the large size of BiBACs and low-copy number in Escherichia coli (DH10B) and Agrobacterium tumefaciens make cloning experiments more difficult than other available binary vector systems. Therefore, a protocol that outlines preparation, modification, and transformation of high-molecular weight (HMW) constructs is advantageous for researchers looking to use BiBACs in plant genomics research. This unit does not cover the cloning of HMW DNA into BiBAC vectors. Researchers looking to clone HMW DNA into BiBACs can refer to Zhang et al. (2012; doi: 10.1038/nprot.2011.456).",

keywords = "Agrobacterium tumefaciens, BiBAC, genetic engineering, transgenes",

author = "Cody, {Jon P.} and Graham, {Nathaniel D} and Birchler, {James A.}",

note = "Funding Information: Research on this topic was supported by grant IOS‐1339198 from the National Science Foundation. Publisher Copyright: {\textcopyright} 2017 John Wiley & Sons, Inc.",

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AU - Graham, Nathaniel D

AU - Birchler, James A.

PY - 2017/12

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N2 - Binary Bacterial Artificial Chromosomes (BiBAC) are large insert cloning vectors that contain the necessary features required for Agrobacterium-mediated transformation. However, the large size of BiBACs and low-copy number in Escherichia coli (DH10B) and Agrobacterium tumefaciens make cloning experiments more difficult than other available binary vector systems. Therefore, a protocol that outlines preparation, modification, and transformation of high-molecular weight (HMW) constructs is advantageous for researchers looking to use BiBACs in plant genomics research. This unit does not cover the cloning of HMW DNA into BiBAC vectors. Researchers looking to clone HMW DNA into BiBACs can refer to Zhang et al. (2012; doi: 10.1038/nprot.2011.456).

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BiBAC Modification and Stable Transfer into Maize (Zea mays) Hi-II Immature Embryos via Agrobacterium-Mediated Transformation

Abstract

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