BiBAC Modification and Stable Transfer into Maize (Zea mays) Hi-II Immature Embryos via Agrobacterium-Mediated Transformation

Jon P. Cody, Nathaniel D Graham, James A. Birchler

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Binary Bacterial Artificial Chromosomes (BiBAC) are large insert cloning vectors that contain the necessary features required for Agrobacterium-mediated transformation. However, the large size of BiBACs and low-copy number in Escherichia coli (DH10B) and Agrobacterium tumefaciens make cloning experiments more difficult than other available binary vector systems. Therefore, a protocol that outlines preparation, modification, and transformation of high-molecular weight (HMW) constructs is advantageous for researchers looking to use BiBACs in plant genomics research. This unit does not cover the cloning of HMW DNA into BiBAC vectors. Researchers looking to clone HMW DNA into BiBACs can refer to Zhang et al. (2012; doi: 10.1038/nprot.2011.456).

Original languageEnglish (US)
Pages (from-to)350-369
Number of pages20
JournalCurrent protocols in plant biology
Volume2
Issue number4
DOIs
StatePublished - Dec 2017

Bibliographical note

Funding Information:
Research on this topic was supported by grant IOS‐1339198 from the National Science Foundation.

Publisher Copyright:
© 2017 John Wiley & Sons, Inc.

Keywords

  • Agrobacterium tumefaciens
  • BiBAC
  • genetic engineering
  • transgenes

Fingerprint

Dive into the research topics of 'BiBAC Modification and Stable Transfer into Maize (Zea mays) Hi-II Immature Embryos via Agrobacterium-Mediated Transformation'. Together they form a unique fingerprint.

Cite this